TL;DR
It is shown that bulk expression can misrepresent where a lncRNA resides in both directions, and that cell-of-origin attribution should precede biomarker claims, and that cell-of-origin attribution should precede biomarker claims.
An lncRNA-aware single-cell analysis framework — retaining all detectable GENCODE v45 lncRNAs during highly variable gene selection — combined with donor-level validation that guards against pseudoreplication is presented, and the pseudotime screen is reported as a negative result.
A single-cell multiomics atlas of the human pancreas is presented, profiling over four million cells and nuclei from 57 donors across fetal development, adult homeostasis, and type 2 diabetes.
Nominating DIO3OS and C9orf139 as candidate survival-associated lncRNAs in GBM is shown, to show how endpoint definition, selection optimism, and external validation determine which lncRNA associations survive scrutiny.
Diffuse Large B-cell lymphoma (DLBCL) is subdivided into the Germinal Center B-cell (GCB) and non-Germinal Center B-cell (non-GCB)/Activated B-cell (ABC) subtypes, with significant clinical, phenotypic, and genetic variability. Investigating DLBCL’s molecular mechanisms and developing tailored treatment strategie...
How genetic variation regulates long non-coding RNA (lncRNA) expression across brain cell types remains poorly understood. A major barrier is the resolution–power trade-off between bulk and single-nucleus expression quantitative trait locus (eQTL) studies. Here, we quantify gene expression from cortical RNA-seq of 2443...
This study established an integrative strategy enabling high confidence cell identity assignment in sparse epigenomic data, and revealed the roles of B cell subtypes in the regulation of tumor immunity.
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