Analytical validation of a modified wild-type blocker qpcr assay for the sensitive DNA-based detection of NPM1 type A in acute myeloid leukemia

Pejman Hamedi-Asl Dariush Hamedi-Asl Shaharbano Rostami Mahmoud Barati Ali Amini Davod Jafari Fatemeh Damerchiloo Rima Manafi Majid Safa

Aug 2026 · Molecular Biology Reports · Vol 53 · 0 citations · 46 references

Medicine

TL;DR

The modified WTB-qPCR assay offers a rapid, highly sensitive, and cost-effective DNA-based method for MRD monitoring in NPM1-A–mutated AML, and its strong analytical performance and straightforward workflow support its implementation as a routine diagnostic tool in clinical laboratories.

DOI PubMed Semantic Scholar

View source

Similar papers

Open access Aug 2026

Integration of PNA-mediated PCR and CRISPR/Cas13a for highly sensitive detection of EGFR T790M mutation in circulating tumor DNA.

The PNA-Cas13a assay enables sensitive and specific detection of EGFR T790M mutations in ctDNA, is readily adaptable to multiple gene loci, and holds promise for clinical monitoring of tumor drug resistance.

Yang Yang, Yali Xie, Li Wang · Molecular and Cellular Probe... · 0 citations

Oct 2026

B-315 Analytical validation and implementation of a high-throughput allele-specific qPCR assay for the PAI-1 4G/5G polymorphism genotyping

Routine genotyping of common variants in many laboratories still relies on labor-intensive methods with limited throughput. Recent advances in qPCR chemistry (e.g., modified fluorescent probes) and automation (e.g., automated liquid handling) enable more efficient and scalable workflows for clinically relevant te...

L. Nery, B. Rodrigues, Y. Costa et al. · Clinical Chemistry · 0 citations

Sep 2026

Development and validation of a quantitative real-time PCR method for determination of residual host DNA in recombinant human C1 esterase inhibitor (rhC1INH).

A quantitative real-time polymerase chain reaction (qPCR)-based method was established and validated for the detection of residual host DNA in recombinant human C1 esterase inhibitor (rhC1INH), a biopharmaceutical produced in the mammary glands of transgenic rabbits. DNA was extracted from rhC1INH samples using a sodiu...

Cong Cong, Xiao-Ping Yu, Tian-Fei Du et al. · Biologicals (Print) · 0 citations

Open access Sep 2026

A SuperSelective primer-based real-time PCR platform for hypersensitive detection of azole resistance in Cryptococcus neoformans

ABSTRACT Cryptococcus neoformans is the leading cause of fungal meningitis with limited treatment options, making early and accurate diagnosis critical for improved patient outcomes. Current diagnostic methods for cryptococcosis rely largely on capsule antigen detection and fungal culture, which are time-consuming and...

S. Pawar, Hong-Lin Xue, Sophia Wang et al. · Microbiology spectrum · 0 citations

Open access Sep 2026

Limit of Blank Variability as a Determinant of Analytical Sensitivity in a DNA-Binding Dye-Based Digital Polymerase Chain Reaction for the Detection of Chlamydia pneumoniae

Digital PCR is considered a highly sensitive technique for detecting low-abundance DNA in samples containing excess background DNA. This is particularly relevant for Chlamydia pneumoniae infections, as this obligatory intracellular bacterium can be disseminated by phagocytes from the respiratory tract throughout the wh...

Theresa Kaudela, Eveliina Taavitsainen-Wahlroos, Leena Hanski · Analytical Chemistry · 0 citations

Open access Sep 2026

An integrated strategy of multiplex qPCR and BclA gene length polymorphism analysis for differentiating Bacillus anthracis wild-type and vaccine strains

ABSTRACT A combined molecular strategy was developed to accurately differentiate wild-type and vaccine strains of Bacillus anthracis. This approach integrates a multiplex TaqMan qPCR assay targeting the BclA, sap, and cap genes with conventional PCR analysis of BclA fragment length polymorphism. The qPCR assay demonstr...

Jia-Qi Yang, Yan Liang, Bing Liang et al. · Microbiology spectrum · 0 citations