Findings show that endogenous metabolism and supplementation timing are deeply interconnected and are critical considerations in CFES optimization, and that metabolomics-informed fed-batch supplementation is a potentially valuable strategy to improve reaction productivity.
Recent advances in fully autonomous ncAA biosynthesis for GCE technology across microbial, zebrafish, and mammalian systems are summarized and emerging applications of these biosynthetic strategies in biological research and therapeutic development are highlighted.
Dan Wu, Mengxi Zhang, Yu Hu et al.· Angewandte Chemie· 0 citations
Over the past 50 years, the Drosophila melanogaster S2 cells have been valued for their ability to synthesize therapeutic molecules at high yield. To further increase protein expression, it is imperative to improve cellular performance, which is intrinsically linked to cell metabolism. Nevertheless, information on S2 m...
M. C. Decarli, D. P. Dos Santos, D. Correia et al.· Biotechnology Journal· 0 citations
Microbial CO₂ assimilation provides a strategy for sustainable biomanufacturing. However, CO₂ reduction requires substantial energy input, while native energy systems are tightly regulated and extensively consumed by endogenous metabolism, limiting assimilation efficiency. Here, we design and construct an orthogonal en...
ABSTRACT Heterologous expression—the expression of genes from one organism into another—is an important approach to produce value-added chemicals such as proteins and metabolites. The main challenge, however, is achieving high production yields that are commercially viable. Approaches used to improve productivity rely...
Adjo E. Kadjo, Galen Ptacek, Barbara I. Adaikpoh et al.· Applied and Environmental Mi...· 0 citations
Biopharmaceuticals, particularly those produced using mammalian cell systems, have reshaped modern therapeutic approaches by enabling the production of complex and efficacious recombinant proteins. This study provides a comprehensive analysis of upstream process development strategies in mammalian cell-based biopharmac...
Buğrahan Regaip Kilinç, Oguzhan Ustun, Neda Abadian et al.· Journal of Balkan Science an...· 0 citations
SUMMARY Over the past two decades, the field of bioorthogonal chemistry has transitioned from emerging to an established cornerstone of scientific inquiry. In parallel, advances in microbial and host–microbe research have highlighted the need for functional approaches that extend beyond genomic and transcriptomic analy...
Grace Z. Wang, C. VanDrisse· Microbiology and Molecular B...· 0 citations
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