Frequency of polymorphic variants of the IFNG (rs2069705) and IL28b (rs12979860) genes depending on the severity of clinical manifestations of Opisthorchis felineus invasion

Abstract

Introduction. The study of the role of cytokines in cellular interactions in liver diseases is currently given great importance. However, studies in this area in patients with Opisthorchis felineus (O. felineus) invasion are still limited. Aim . To study the relationship between laboratory and instrumental manifestations of pathology with polymorphisms of the genes IFNG (rs2069705) and IL28b (rs12979860) in patients with O. felineus invasion. Materials and methods . A total of 360 patients with O. felineus invasion (170 men and 192 women, mean age 42.1 ± 0.7 years) and 124 control group individuals (65 men and 59 women, mean age 48.3 ± 1.1 years) were examined. O. felineus invasion was diagnosed using duodenal bile microscopy and coproovoscopy. All patients underwent complete blood count, biochemical blood test, abdominal ultrasound, and liver elastometry with liver fibrosis assessment using the METAVIR scale. Genotyping of single nucleotide polymorphisms of the genes IFNG (rs2069705) and IL28b (rs12979860) was performed using real-time PCR. Results. In patients with O. felineus invasion, liver fibrosis was associated with the heterozygous TC genotype for the rs2069705 IFNG polymorphism. Elevated alkaline phosphatase levels and an increased proportion of blood eosinophils were more prevalent in patients with the homozygous CC genotype of the rs2069705 IFNG polymorphism. We found no association between the rs12979860 IL28b polymorphism and manifestations of O. felineus invasion. Conclusion. From our point of view, patients with opisthorchiasis who have the rs2069705 IFNG polymorphism should be identified during dispensary observation as a risk group to prevent the development of complications of parasitic invasion, which include liver fibrosis and hepatocellular carcinoma.