Combinatorial deep mutational scanning uncovers protein superbinders and molecular determinants of epistasis.
Traditional deep mutational scanning (DMS) encodes every single amino-acid substitution from a wild-type sequence. We hypothesize that combinatorial DMS (CDMS) libraries, incorporating all mutations in all combinations, can enable the discovery of high-affinity protein (super)binders by capturing epistatic, non-linear...
Ming-Xuan Jiang, Mo-Han Sun, Nuo Cheng et al.
· Molecules and Cells · 0 citations