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Author

James Aaron Kraemer

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#gene editing Open access Sep 2026

Evaluation of Alcalase pretreatment for Chlamydomonas reinhardtii CRISPR knock-in

We wanted to use CRISPR-Cas9-mediated homology-directed repair (HDR) in C. reinhardtii to insert genomic landing pads as part of a larger project. In cell-walled algae, delivering Cas9 ribonucleoproteins (RNPs) and donor DNA requires first digesting that wall. The standard approach uses autolysin, a gamete-derived prot...

Daniel Caddell, Raymond Futia, James Aaron Kraemer · 0 citations
#protein folding Open access Sep 2026

Joint steering of protein generation across multiple target properties

Ideally, protein design could optimize several properties at once while ensuring the protein satisfies basic biophysical constraints such as folding, stability, solubility, and expression. However, most guided generation methods optimize only one or two simple objectives. We developed a workflow that uses predictive mo...

Prachee Avasthi, Josie Bircher, James Aaron Kraemer et al. · 0 citations

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