In many cells, the nuclear envelope (NE) must be reassembled after mitosis, and holes in the nuclear membrane must be sealed. During NE assembly, the NE-specific adaptor, Cmp7, recruits/activates endosomal sorting complex required for transport (ESCRT)-III proteins to mediate NE sealing. However, recent evidence sugges...
Emma M. Sydir, M. Farra, Abigail L. Whitford et al.· Journal of Cell Biology· 0 citations
KRAS is the dominant oncogene in pancreatic ductal adenocarcinoma (PDAC), mutated in ∼90% of tumors, and required for tumor initiation and tumor maintenance. The overall 5-year survival rate of PDAC remains a dismal ∼10%, underscoring an unmet need to develop novel therapies to ultimately improve patients’ outcomes....
Jonathan M. DeLiberty, Fan-Yi Kong, Qi-Jia Yu et al.· Cancer Research· 0 citations
This work shows that the human ER-resident RNF185 ubiquitin ligase complex destabilizes a small but specific set of membrane proteins that span all three ERAD branches, identifying RNF185-destabilized membrane proteins with distinct aberrancies that collectively encompass all canonical ERAD substrate classifications an...
Haruka Chino, Isabella M. Ruiz, Joshua H. Corbo et al.· bioRxiv· 0 citations
Membrane fluidity depends on unsaturated acyl chains that are generated in Saccharomyces cerevisiae by the desaturase Ole1, whose expression is primarily under the control of the transcription factor Mga2. Here, we show that the endoplasmic reticulum-anchored Mga2 precursor is ubiquitinated by the E3 ligase Rsp5 and th...
Neng Wan, Jeremi Kuklewicz, João A. Paulo et al.· bioRxiv· 0 citations
Lineage-defining transcription factors are key oncogenic drivers but remain difficult to target pharmacologically due to the absence of ligandable pockets. The molecular rules governing substrate recognition by large HECT ubiquitin ligases also remain incompletely understood, limiting efforts to exploit these enzymes f...
Keita Masuzawa, Kevin D. Dong, Calvin XiaoYang Hu et al.· Genes & Development· 0 citations
It is shown that DNAJC13 forms an unexpected antiparallel homodimeric architecture involving distinctive symmetrical interactions between composite IWN1 and α-solenoid ARM2 domains in each protomer, which provides a structural and mechanistic framework for understanding DNAJC13 function in recycling endosome control.
Tao Fu, Chan Lee, Frances V. Hundley et al.· bioRxiv· 0 citations
Improvements enabling quantification of site-specific modifications, including post-translational modifications and covalent compound-protein interactions spanning diverse pathways are described.
Steven R. Shuken, Geordon A. Frere, Charlotte R. Beard et al.· Nature Communications· 0 citations
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