Cytokinesis requires coordinated signaling to ensure the accurate physical separation of daughter cells. Calcineurin (CN), a conserved Ca²⁺/calmodulin-dependent phosphatase, is required for cytokinesis in organisms ranging from yeast to humans, yet how CN is regulated at the division site and the substrates through whi...
Alaina H. Willet, Jun-Song Chen, Qing Yu et al.· bioRxiv· 0 citations
Membrane fluidity depends on unsaturated acyl chains that are generated in Saccharomyces cerevisiae by the desaturase Ole1, whose expression is primarily under the control of the transcription factor Mga2. Here, we show that the endoplasmic reticulum-anchored Mga2 precursor is ubiquitinated by the E3 ligase Rsp5 and th...
Neng Wan, Jeremi Kuklewicz, João A. Paulo et al.· bioRxiv· 0 citations
Lineage-defining transcription factors are key oncogenic drivers but remain difficult to target pharmacologically due to the absence of ligandable pockets. The molecular rules governing substrate recognition by large HECT ubiquitin ligases also remain incompletely understood, limiting efforts to exploit these enzymes f...
Keita Masuzawa, Kevin D. Dong, Calvin XiaoYang Hu et al.· Genes & Development· 0 citations
KinoPlex, a computational framework that integrates predicted protein structures and kinase recognition motifs to assign phosphorylation potential and kinase specificity to all serine/threonine/tyrosine residues, is presented.
David Vanderwall, Edward L. Huttlin, J. Mintseris et al.· Nature Biotechnology· 0 citations
Improvements enabling quantification of site-specific modifications, including post-translational modifications and covalent compound-protein interactions spanning diverse pathways are described.
Steven R. Shuken, Geordon A. Frere, Charlotte R. Beard et al.· Nature Communications· 0 citations
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