gene editing

Benchmarking emerging chromatographic platforms for mRNA-LNP characterization and scalable process enablement

Lipid nanoparticles (LNPs) are now the leading delivery platform for nucleic acid therapeutics, supporting applications from prophylactic mRNA vaccines to in vivo gene editing, in vivo CAR-T, protein replacement, and personalized cancer immunotherapy. As formulations incorporate novel ionizable lipids, targeting ligand...

Ultrasound-responsive nanotheranostic systems for glioblastoma: Materials evolution, design strategies and clinical translation perspectives

Glioblastoma (GBM) remains one of the most aggressive primary brain malignancies, characterized by a poor prognosis and limited therapeutic efficacy due to the restrictive nature of the blood–brain barrier (BBB), marked tumour heterogeneity, and the rapid development of therapeutic resistance. These challenges signific...

Regulation of Human Cellular and Gene Therapeutic Products Incorporating Genome Editing Technologies in India

The legal framework and the guidelines issued by the regulatory bodies must secure the safe and ethical application of these products, while timely approval is equally necessary if the unmet need for these lifesaving therapies is to be met.

What´s in a name: the issue with regulatory definitions

The title refers to a line from William Shakespeare’s Romeo and Juliet to suggest that names are unimportant 1 . While this may hold true in the context of human equality, clear terminology and definitions are essential for legal and regulatory purposes. Clear, unambiguous definitions reduce discretionary interpretatio...

Aromatic Rice Created through CRISPRCas9-Mediated BADH2 Gene Editing

Integrating molecular engineering and artificial intelligence to overcome clinical and manufacturing challenges in CAR-T therapy

This review presents an integrated perspective on molecular-level CAR-T cell engineering and AI-based computational and manufacturing systems, rather than treating them as separate domains, and examines the role of AI in both developing and processing cell-based CAR-T development of molecular constructs.

Belajar Tentang Agent Biologis -- Agent Biologis: A to Z

Mengapa buku ini dibutuhkan Terapi biologis berkembang pada pertemuan biologi molekuler, imunologi, genetika, biomaterial, manufaktur, uji klinis, regulasi, dan etik. Kompleksitas ini sering menimbulkan dua kesalahan ekstrem: menolak inovasi hanya karena belum familiar, atau menerima klaim hanya karena teknologi tampak...

Modern approaches and future prospects in lettuce breeding for controlled environment agriculture

Controlled environment agriculture (CEA) is expanding rapidly as a climate-resilient approach for year-round lettuce production near consumption centers. However, despite its supply chain advantages and high productivity, the economic viability and scalability of CEA-grown lettuce remain constrained by the continued re...

Understanding the Specification Mechanism of the Megaspore Mother Cell in Arabidopsis

The megaspore mother cell (MMC) is the founding cell of the plant female germline, and its specification is a critical step in sexual reproduction of flowering plants. Recent advances in single-cell transcriptomics, live imaging, and gene editing have significantly advanced our understanding of the molecular mechanisms...

Optimizing CRISPR/Cas9 mutagenesis in Drosophila dendritic arborization neurons to avoid cytotoxicity

The use of CRISPR mutagenesis in Drosophila da neurons is refined through titration of Gal4/UAS-mediated Cas9 expression using existing uCas9 transgenes, a feasible and flexible approach that may be useful for other labs encountering Cas9 cytotoxicity in their own model systems.

Integrating genomics, multi-omics, CRISPR and speed breeding for stress-resilient vegetable legume improvement

This review synthesizes advances in QTL mapping, genome-wide association studies, transcriptomics, metabolomics, and CRISPR-based functional genomics that have identified key regulators and pathways underlying resistance to major biotic and abiotic stresses into a systems-breeding framework to bridge the gap between ge...

Critical role for the TGF-β1/mTORC1 signaling axis in defining the transcriptional identity of CTHRC1+ pathologic fibroblasts

It is concluded that mTORC1 plays a key role in shaping the transcriptional identity of these fibroblasts, with implications for therapeutic inhibition of mTORC1 in fibrosis and cancer.