gene editing

Evaluating Computational Tools for CRISPR-Cas9 Design: A Case Study on the TP53 Gene

Comparative performance of CRISPR epigenome gene activation: a systematic review on potency and temporal dynamics in mammalian models

Epigenome editing refers to the targeted alteration of gene regulatory states without altering the underlying DNA sequence in a heritable or not manner, directly or indirectly. Specifically, CRISPR activation (CRISPRa) is a epigenome editing system based on the activity of a Cas9 that lacks endonuclease activity (dCas9...

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This paper introduces a novel system for personalized gene editing based on dynamic gene expression modulation, leveraging biological information to tailor gene expression to individual characteristics and environmental factors. Traditional gene editing methods primarily focus on modifying genes, while this system aims...

Beyond Viral Suppression: Biological, Clinical, and Social Challenges in HIV Control

While antiretroviral therapy (ART) has transformed HIV into a manageable chronic condition, the virus remains a persistent global health challenge. This review connects the biological foundations of HIV, including its zoonotic origins, the pandemic expansion of HIV-1 group M, and the genetic diversity generated by rapi...

Comprehensive Comparative Evaluation and Consensus Strategy for CRISPR/Cas9 gRNA Design Targeting the TaDREB1 Gene in Bread Wheat (Triticum aestivum)

SAVI: molecular mechanisms, clinical spectrum and precision medicine approaches beyond type-I IFN

The transition from immune - to a mutational-perspective, provides a foundational framework to optimize diagnostic and therapeutic strategies for precision medicine in SAVI patients.

From Design to Single-Cell Cloning: A Complete RNP-Based CRISPR-Cas9 Protocol for Precision Gene Correction in Human iPSCs.

A systematic, comprehensive and efficient workflow combining generation, genotyping and expansion of high-quality monoclonal iPSC lines following CRISPR-Cas9 genome editing, which provides a robust and high-throughput framework for the reliable production of monoclonal genome-edited iPSCs, thereby advancing translation...

Molecular Scissors With a Search Bar: A Historical Development Review of CRISPR and the Editing of the Genome

This article presents a narrative review of CRISPR and the Editing of the Genome in the context of Biology. The literature on this topic has expanded substantially over recent decades, yet it remains fragmented across subfields, methods, and national research traditions. Drawing on an interpretive synthesis of represen...

Next-generation vascular organoids: multi-lineage and immune integration for disease modeling and regenerative medicine

An emerging paradigm shift toward third-generation, multi-lineage, and immune-competent vascular organoids is synthesized, with emphasis on fluidic shear stress in microfluidic platforms, the spatial precision afforded by three-dimensional bioprinting, and multiplex CRISPR gene editing.

Editorial: The safety assessment of novel foods and new nutrient sources

An important theme that emerged from this Research Topic is the toxicological safety assessment of novel foods including genotoxicity and repeated dose toxicity. Schreppler et al., employed a standard battery of in vitro genotoxicity tests (bacterial reverse mutation assay, in vitro mouse lymphoma TK gene mutation assa...

基于生物网络的动态拓扑优化

This paper explores the application of dynamic topology optimization techniques to biological networks, aiming to enhance drug discovery and gene editing. Biological networks, complex systems of interconnected nodes and edges, are increasingly utilized in various fields. Traditional optimization methods often rely on f...

基于生物网络的动态拓扑优化

This paper explores the application of dynamic topology optimization techniques to biological networks, aiming to enhance drug discovery and gene editing. Biological networks, complex systems of interconnected nodes and edges, are increasingly utilized in various fields. Traditional optimization methods often rely on f...