gene editing

From single-pathway cascade to network pathophysiology: how genetically engineered mouse models reshaped our understanding of pancreatitis

Evidence is synthesized from genetically engineered mouse models that enhanced trypsinogen autoactivation alone is sufficient for spontaneous disease, that NF-κB-driven inflammation can be initiated independently of trypsin, and that loss of autophagy alone disrupts acinar homeostasis, which reframe pancreatitis as a network pathophysiology with two coupled layers.

Genomics-assisted Improvement of Resistance to Insect Pests, Diseases and Nematodes in Tomato: A Critical Appraisal of Discovery, Durability and Deployment

Whether, and where, genomic technologies have altered breeding outcomes rather than merely accelerating gene discovery is examined, and the available evidence indicates that genomic resources have substantially improved the resolution of resistance discovery and the precision of marker-assisted introgression, but have not yet demonstrably improved durability.

Correction to “Therapeutic Gene Editing: DNA Repair Pathways, Emerging Editors, and Clinical Progress”

Genomic architecture of hereditary defects in beef cattle populations: from molecular diagnostics to innovative therapy approaches (review)

Recommendations are formulated for building a genetic health management system in beef cattle breeding, based on mandatory DNA testing, inbreeding control, accounting for pleiotropic effects in selection, and the integration of advanced biotechnologies.

CRISPR/Cas9-Induced Dwarfism in Barley: Impacts on Yield-Related Traits and Root Architecture

These results provide new insights into HvDEP1′s role in both shoot and root systems and demonstrate that precise CRISPR/Cas9-mediated editing can rapidly introduce dwarfism while revealing trade-offs in other agronomic traits.

Cryptomeria japonica var. sinensis Miquel (Chinese Cedar): A Comprehensive Review of Its Biology, Phytochemistry, Biotechnology, and Utilization

This review provides a comprehensive foundation for understanding the biology, phytochemistry, biotechnology, and utilization of this invaluable botanical resource, offering a strategic roadmap for its sustainable management and commercial valorization.

Navigating Quality, Safety, and Analytical Testing Frameworks for Viral Vector–Based Gene Editing Therapies

This article aims to provide a working framework for verifying the potency, genomic integrity, and clinical safety of vector-based gene therapies—one intended to be useful both to laboratories developing these products and to those responsible for regulating them.

Comparative chloroplast genomics of six Bupleurum (Apiaceae) accessions: candidate barcodes, phylogeny based on available plastomes, and candidate RNA-editing sites

The whole-plastome phylogeny recovered Bupleurum as monophyletic relative to Chamaesium, and only one of seven multi-accession operational binomial groups was monophyletic, and only one showed a positive local barcode gap.

Long-term in vivo CRISPR/Cas9 gene editing in liver triggers host immunity and clonal mutations in DNA damage response genes beyond off-target effects.

Integrating Multi-Omics in Aquaculture: From Genetic Gains to Sustainable Aquaculture

This review outlines foundational applications of omics in aquaculture and highlights the characteristics and current applications of distinct omics approaches, and summarizes three core application domains of omics in aquaculture.

Establishment of a CRISPR/Cas9-mediated system for targeted editing of the MFS gene in mint

A substantial decrease in menthofuran content in the essential oil of the edited line #10 compared to the wild-type control is revealed, thereby demonstrating a viable strategy for improving mint essential oil quality through genome-editing.

Mutation-induced phenotypic plasticity drives formation of a pro-metastatic tumour-intrinsic niche

It is argued that formation of a tumour-intrinsic niche is a prerequisite for BRAF-mutant CRC seeding to distant organs and that interference with niche formation may help avoid metastatic relapse.