gene editing

EDIT-B consortium: an external pan-European validation of an innovative machine learning-based test for bipolar disorder diagnosis.

This study confirms the diagnostic performances of EDIT-B showing that A-to-I RNA editing coupled with ML methods is a reliable approach to distinguish BD from MDD, a significant advance towards precision psychiatry.

Transcription factor TBX2 is a key regulator of vascular calcification.

BACKGROUND AND AIMS Vascular calcification predicts cardiovascular mortality more strongly than traditional risk factors, yet its molecular basis remains poorly understood. The burden is greatest in chronic kidney disease (CKD), where disordered mineral metabolism accelerates arterial mineral deposition. METHODS Cros...

Engineering Oilseed Crops for Sustainable Production of Functional Fatty Acids

Plant oils are renewable sources of fatty acids with diverse biological, nutritional, and industrial applications. Our research focuses on developing plant-based production systems for two valuable types of fatty acids: hydroxy fatty acids (HFAs) and high-oleic oils. Using metabolic engineering and CRISPR/Cas9 genome e...

Engineering Oilseed Crops for Sustainable Production of Functional Fatty Acids

Plant oils are renewable sources of fatty acids with diverse biological, nutritional, and industrial applications. Our research focuses on developing plant-based production systems for two valuable types of fatty acids: hydroxy fatty acids (HFAs) and high-oleic oils. Using metabolic engineering and CRISPR/Cas9 genome e...

Reduced cellulose crystallinity in gene-edited poplar modulates structure–property relationships in cellulose nanofibrils and nanocrystals

Generation of DFNA5-deficient chickens reveals a species-specific role of pyroptosis in avian antiviral immunity

This work establishes the first germline-edited heterozygous DFNA5-mutant chicken model using primordial germ cell mediated genome editing and demonstrates that genetic disruption of DFNA5 markedly attenuates Raptinal-induced pyroptosis and reduces IL-1β and IL-18 secretion.

Genetic Counseling

This review aims to provide a brief historical overview of genetic counseling and its evolution, addressing the qualified professionals and clinical requirements necessary to obtain a diagnosis and post-test guidance across different stages of patients' lives. Recent advances in the understanding of genetic diseases an...

Elucidating Gene Functions in Congenital Heart Disease

Genome editing, CRISPR screening, base and prime editing, regulatory-element assays, and inducible protein-depletion systems now allow increasingly precise and high-throughput characterization of coding and noncoding variants, enabling a transition in CHD research from descriptive genomics to integrated, multi-model ap...

Exosomes and Extracellular Vesicles in Oncology: Next-Generation Smart Nanocarriers for Precision Cancer Therapy

Exosomes are an evolved sub-type of extracellular vesicles (EVs) that have gained popularity in recent years as a novel biological nanocarrier system for targeted precision therapy in cancer owing to their excellent biocompatibility, low immunogenicity, and high stability. While other artificial systems can only delive...

Physiological and Molecular Mechanisms of Tree Responses to Combined Drought and Low-Nitrogen Stress: A Review

Drought and low nitrogen often co-occur in forests, yet their combined effects cannot be inferred reliably from single-stress studies. This review synthesizes physiological and molecular evidence for tree responses to combined drought and low nitrogen at four levels, namely stress perception, signal transduction, metab...

Impact of Sphingolipid Metabolizing Enzyme β-Galactosylceramidase on Mitochondrial Sphingolipid Profile and Energetic Metabolism in Human Melanoma Cells

Background/Objectives: Mitochondrial plasticity, reflected by the capacity of cancer cells to shift between oxidative phosphorylation and glycolytic metabolism in response to genetic alterations and changes in the tumor microenvironment, represents a key feature of melanoma progression. Sphingolipids contribute to the...

Immunomodulatory strategies for ex vivo immune cell expansion in adoptive cell therapy: translational and regulatory considerations for clinical manufacturing

Ex vivo expansion is a fundamental yet challenging step in the manufacturing of adoptive cell therapies (ACT), as therapeutic success relies on generating enough functional immune cells while maintaining their phenotype, metabolic fitness, and persistence. This review provides a comprehensive overview of immunomodulato...