gene editing

🌱 REGENERATION, REPROGRAMMING & REJUVENATION ENGINEERING AT THE LIMIT Stem Cells, Senescence, Tissue Regeneration, Epigenetic Reset, Gene Engineering, and the Emerging Science of Rejuvenation Control

🌱 REGENERATION, REPROGRAMMING & REJUVENATION ENGINEERING AT THE LIMIT Stem Cells, Senescence, Tissue Regeneration, Epigenetic Reset, Gene Engineering, and the Emerging Science of Rejuvenation Control How far can rejuvenation proceed without destroying identity? This flagship research volume explores one of the most di...

Mitochondria‐Targeted Nanotherapeutics: A Promising Strategy in Modulating Mitochondrial Function, Transfer, and Transplantation

This review provides a structured synthesis that spans from the biological basis of mitochondrial function and transfer to the broad applications of nanomaterials in mitochondria‐targeted therapies, with a dedicated analysis of their roles in mitochondrial targeting, functional modulation, and gene editing.

🌱 REGENERATION, REPROGRAMMING & REJUVENATION ENGINEERING AT THE LIMIT Stem Cells, Senescence, Tissue Regeneration, Epigenetic Reset, Gene Engineering, and the Emerging Science of Rejuvenation Control

🌱 REGENERATION, REPROGRAMMING & REJUVENATION ENGINEERING AT THE LIMIT Stem Cells, Senescence, Tissue Regeneration, Epigenetic Reset, Gene Engineering, and the Emerging Science of Rejuvenation Control How far can rejuvenation proceed without destroying identity? This flagship research volume explores one of the most di...

Clinical Translation of Engineered Cell and Gene Therapies for HIV Cure: A Systematic Review and Target-Product-Profile Gap Analysis of Human Interventional Studies

This systematic review evaluates the clinical translation of engineered cell and gene therapies developed for HIV cure, sustained remission, or durable antiretroviral therapy (ART)-free control. The review focuses on human interventional studies evaluating genetically engineered therapeutic products, including gene-edi...

Promoter-Targeted CRISPR Correction of PMP22 Gene Dosage Restores Schwann Cell Homeostasis in Charcot-Marie-Tooth Disease Type 1A.

Collectively, patient-anchored, promoter-targeted genome editing can potentially correct pathogenic PMP22 dosage while preserving basal expression, establishing a translational iPSC-based framework for treating dosage-sensitive demyelinating neuropathies such as CMT1A.

Precision reactivation of fetal hemoglobin: expanding the gene-editing landscape for β-thalassemia

CRISPR-Cas9 Genome Editing in Mesorhizobium ciceri Through nodC Disruption for Chickpea Symbiosis Studies.

A streamlined genome-editing strategy using a broad-host-range Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-associated protein 9 (Cas9) system adapted for M. ciceri is described, providing an efficient and reproducible framework for precise gene disruption and functional genomics studies in Mesorh...

Safety Challenges and Emerging Solutions in Regenerative Medicine: Tissue Engineering, Cell Therapy, and Gene Therapy.

This commentary highlights key safety concerns across major regenerative medicine categories and emerging solutions to address these risks, along with technological and regulatory approaches in development to improve product safety and consistency.

In vitro validation of a CRISPR/Cas9 guide RNA targeting COL1A1 gene by cleavage assay.

Functional validation of the gRNAs designed to target exon 2 of the COL1A1 gene is confirmed through in vitro cleavage activity and support its candidate selection for subsequent cellular studies targeting COL1A1, representing an initial step toward future knockout-style cellular models of Osteogenesis Imperfecta.

Comparative evaluation of germ cell-specific promoters identifies robust drivers of transgene expression during mouse spermatogenesis

The findings suggest that the Ldhc and Dazl promoters used in this study are robust and are expressed in testicular germ cells, which can be used to study gene regulation during spermatogenesis to understand testicular physiology and male fertility.

Emerging roles of microproteins in plant developmental regulation, stress adaptation and crop improvement

This review, primarily focuses on classical plant miPs that modulate protein activity through dominant-negative PPI, while also briefly discussed associated classes of small regulatory peptides, where they intersect functionally with miP-mediated regulation.

Introgressive hybridization fuels local adaptation in oakleaf butterflies

Hybridization may have multiple impacts on speciation and adaptation. Yet the evidence of adaptive introgression events that have historically occurred between nonradiating or established species is still lacking. The oakleaf butterflies in the genus Kallima include multiple sympatric and incipient species with a kno...