gene editing

CRYSPER and CUREIPES: two new molecular tools enabling analysis of RNA splicing and C-to-U RNA editing-inducible protein expression at single cell resolution

CUREIPES was developed and is the first molecular tool that enables human Apobec1-dependent C-to-U RNA editing-inducible protein expression, which can be used to counteract pathological alteration of C-to-U RNA editing through cell-autonomous expression of editing-regulatory proteins, or of any other protein of interes...

Microalgal proteins as sustainable alternatives: CRISPR opportunities and challenges.

This review systematically summarizes the comparative advantages, application prospects, and industrialization challenges of microalgal protein as an alternative protein source and provides an integrated framework for the low-cost, scalable, and sustainable production of microalgal alternative protein.

Molecular Mechanisms of WRKY Transcription Factors Integrating Environmental Signals to Regulate Flowering Time in Brassica Crops.

This review summarizes the structural, evolutionary, and functional characteristics of WRKY transcription factors involved in flowering regulation in Brassica species and demonstrates substantial expansion of WRKY gene families through genome triplication and polyploidization.

Characterization of Cellular Alterations in a Novel NAGLU Enzyme-Deficient Cellular Model Generated from U-87MG Cell Line

A glial cell model deficient in the NAGLU enzyme via CRISPR/Cas9 editing system in the U-87MG cell line exhibited typical traits for MPS IIIB, including decreased enzyme activity and mitochondrial mass, as well as increased lysosomal mass, neutral lipids, total glycosaminoglycans, and total reactive oxygen species.

Gene editing to improve stress resilience in wheat

Water deficit, salinity, and low-temperature stresses negatively affect wheat production worldwide, underscoring the need to develop stress-tolerant wheat varieties. This can be achieved by modifying the stress response mechanisms and regulations in plants. The recent DNA-editing approach, CRISPR/Cas editing, can accur...

Efficient transgenesis and homology-directed gene targeting in monolayers of primary human small intestinal and colonic epithelial stem cells

CanProTarget

A residue-resolved prioritization platform for covalent cancer drug discovery. CanProTarget integrates cancer gene dependencies, small-molecule covalent ligand engagement measured by chemoproteomics, and base-editing evidence for individual cysteines, keeping each layer at the resolution it is measured at.

Therapeutic reprogramming of natural killer cells for solid tumors: expanding frontiers into rare and ultra-rare malignancies

Progress in NK cell therapy across solid tumors provides a framework for extending increasingly adaptable and scalable immunotherapeutic platforms to rare and ultra-rare malignancies, with the potential to expand treatment opportunities for patient populations that have historically had limited therapeutic options.

Protein language models predict artemisinin partial resistance and prioritize genetic modifiers in Plasmodium falciparum

Translational Cardiology in Genetic Cardiomyopathies

Translational cardiology applied to genetic cardiomyopathies is a rapidly evolving field that bridges molecular research and clinical practice through a “bench-to-bedside and back” paradigm. Hereditary cardiomyopathies, once classified primarily based on clinical phenotype, are now increasingly understood at the molecu...

Biotechnological Advances and Multi‐Omics Insights for Fruit Quality, Postharvest Biology, and Genetic Improvement in Litchi ( Litchi chinensis Sonn.)

This review critically synthesizes current progress in litchi biotechnology, encompassing chromosome‐scale genome assembly, molecular marker systems, SNP resources, and functional genomics studies that have identified major regulatory genes and transcriptional networks associated with flowering, pigmentation, senescenc...

Bactomics HybAs v8.4-lite

This release is a major workflow upgrade from the legacy 8.4-lite Snakefile to a batch-capable, provenance-aware, benchmarked, validation-enabled, report-ready HybAs implementation. The new workflow preserves the core hybrid assembly logic while substantially improving execution control, reproducibility, taxonomic deci...