gene editing

EDITING OUT CARDIOVASCULAR RISK: A NARRATIVE REVIEW OF GENE-EDITING APPROACHES TO PCSK9

Early clinical and preclinical evidence supports the feasibility of PCSK9 gene editing, and compares CRISPR-Cas9 nuclease knockout, base editing, and epigenetic silencing with established antibody and siRNA classes, focusing on efficacy, durability, and safety.

Does adjusting for tumor proliferation bias prognostic gene expression estimates? Preregistered analysis code, decision rules and results

R analysis code, preregistered decision rules, result files, run logs and figures for a study of what omitting tumor proliferation does to prognostic gene expression estimates. For every expressed gene in a cohort, two Cox models are fitted for overall survival, one adjusted for age, sex and stage and one that adds a s...

Supporting data for “Comparative Genomics in the genus Caenorhabditis and the Discovery of a Novel Toxin-Antidote System in C. elegans”

This dataset accompanies the thesis “Comparative Genomics in the genus Caenorhabditis and the Discovery of a Novel Toxin-Antidote System in C. elegans.” It contains 37 files documenting the comparative genomic analyses and experimental work presented in the thesis. Main and supplementary figures cover genetic mapping,...

Stargardt disease: clinical studies over the past 5 years—a systematic review

Although STGD1 remains a challenging condition, new therapies in this review have shown potential in clinical trials, even though challenges such as off-target effects and DNA repair errors remain.

CRISPR/Cas9-mediated multicopy δ-site integration in Saccharomyces cerevisiae via Cas9 expression

Editorial: Education in synthetic biology

Synthetic Biology (SB) has emerged as a highly innovative field over the last two decades, bringing with it a vast array of tools, objectives, and significant recognition within the broader scientific community. As the field has grown, it has attracted researchers from a wide range of disciplines, including biology, bi...

RsHSFA3 of Rhododendron simsii confers plant heat tolerance by molecular regulation.

Physiological measurements demonstrated that RsHSFA3 positively regulates heat tolerance by boosting reactive oxygen species (ROS) scavenging capacity and preserving cell membrane stability, and synergistically enhances heat tolerance in R. simsii via activating the expression of heat shock proteins and facilitating RO...

Modeling Liver Cancer Molecular and Histological Features by CRISPR Editing of Porcine Hepatocytes.

Through ex vivo CRISPR editing of clinically relevant genes in porcine hepatocytes, ex vivo CRISPR editing of clinically relevant genes in porcine hepatocytes established cell and animal models that recapitulate key molecular and histological features of human HCC.

Adult-onset deficiency of β-klotho in the liver reveals a novel role in lipid homeostasis.

Using an adult-onset liver-specific knockout model, KLB function is disentangled from developmental confounders and established KLB as a critical regulator of adult lipid and lipoprotein homeostasis.

Editing Susceptibility Genes for Broad-Spectrum Resistance in Plants: From Passive Knockout to Active Design.

This Opinion article argues that susceptibility gene editing is shifting from passive knockout to active design. We propose three strategies—targeting conserved host nodes, synergizing S gene knockout with R gene pyramiding and tailored precision editing—to achieve broad‐spectrum resistance without yield penalties.

Engineering silkworm-spider composite silk via the fibroin light chain fusion system in Bombyx mori.

This study employed targeted genome editing to enable the biosynthetic production of spider silk proteins in silkworm silk gland bioreactors and provided a promising strategy for the biofabrication of silkworm-spider composite silk.

Multi-omics integration and artificial intelligence for the conservation and utilization of local chicken genetic resources

Local chicken genetic resources (LCGRs), representing a unique gene pool shaped by millennia of natural and artificial selection, not only sustain the supply of high-quality protein but also serve as useful biological models for deciphering the evolution of complex traits and environmental adaptability. However, extens...