gene editing

ZmWGSL2 Is a Candidate Chloroplast-Localized PPR Gene Associated with Chloroplast Development and Leaf-Colour Formation in Maize

The characterized wgsl2-0, a stable white-green striped leaf mutant isolated from an ethyl methanesulfonate-mutagenized RP125 population of maize, provides strong genetic evidence for ZmWGSL2 as the most likely candidate gene associated with chloroplast development and leaf-colour formation in maize.

Gene editing technologies in poultry: advances, applications, and future prospects

SUMMARYThe chicken is an important model organism for research and a major global source of animal protein (eggs and meat). Recently developed genome-editing technologies have allowed the production of genetically engineered animals for agriculture and food production. Genome editing in chicken is a promising approach...

CRISPR/Cas9 Gene Editing of OsSD1 and OsDEP1 Improves Plant Architecture and Yield in Indigenous Black Rice Cultivar Chakhao Amubi

Rice ( Oryza sativa L.) sustains over half of the global population. The black rice landraces of North-East India are agronomically limited by tall stature, lodging, and low yield. We employed CRISPR/Cas9 -mediated multiplex genome editing to improve the elite aromatic black rice cultivar Chakhao Amubi by targeting thr...

Chromosome Editing Using the Lambda Red Recombinase System in Chlamydia trachomatis and Chlamydia muridarum.

Genome edited sheep generated by zygote electroporation of prime editor ribonucleoproteins

Prime editing (PE) enables precise genome modifications without double-strand breaks, yet its application in large mammals has been hindered by low efficiency and the technical bottlenecks of zygote microinjection. This study aimed to establish a scalable PE platform in sheep via the direct electroporation of a PE3 RNP...

Genome editing frontiers for developing abiotic stress tolerant and climate resilient crops

Gene editing applications, opportunities and challenges for crop improvement in Africa a review

Gene editing, particularly CRISPR/Cas-based new genomic techniques (NGTs), offers significant opportunities for accelerated crop improvement in developing African countries, ensuring food security, livelihoods, climate resilience, and economic growth. This review critically evaluates the scientific basis, applications,...

Hypertrophic Wasp Stem Cell Bio-Hybrid Muscle Actuator Powered and Cryogenically Cooled by Ethanol Solution

A novel theoretical concept in bio-hybrid robotics and tissue engineering: a macroscopic biological muscle actuator engineered from the stem cells of the Oriental Hornet (Vespa orientalis). The system utilizes CRISPR-Cas9 genome editing to multiply the Alcohol Dehydrogenase (NADP+) gene for absolute alcohol immunity, c...

Language-Model-Based Detection of Genetic Editing in Bacteria

A transformer-encoder-based machine-learning classifier that, instead of analyzing words in sentences, models gene families in genomes, provides a scalable method for identifying engineered sequences without relying on specific marker genes, with potential applications in biosecurity, agriculture, GMO regulation and mo...

Measurement reliability bounds functional benchmarks and relocates where variant effect prediction fails

Background Variant effect predictors are increasingly benchmarked against multiplexed assays of variant effect (MAVEs) rather than clinical labels, which removes label circularity but introduces a new problem: a correlation against a measurement cannot exceed the measurement’s own reproducibility, and precision varies...

Multiscale transcriptomic analysis separates epithelial identity loss from FN1-associated stromal remodeling in ulcerative colitis: reproducibility package

This repository contains the reproducibility materials supporting the manuscript “Multiscale transcriptomic analysis separates epithelial identity loss from FN1-associated stromal remodeling in ulcerative colitis.” The archive includes analysis and figure-generation scripts, frozen and processed source tables used for...

Integrated Phenomic and Transcriptomic Network Analysis Reveals Key Regulators of Drought Tolerance in OsC2H2-Edited Rice

Drought poses a major threat to rice ( Oryza sativa ) production, causing severe yield losses and jeopardizing yield stability. C2H2 Jumonji domain–containing zinc-finger proteins play diverse roles in plant growth, development, and abiotic-stress responses through regulation of gene expression and chromatin-associated...