gene editing

Multiscale transcriptomic analysis separates epithelial identity loss from FN1-associated stromal remodeling in ulcerative colitis: reproducibility package

This repository contains the reproducibility materials supporting the manuscript “Multiscale transcriptomic analysis separates epithelial identity loss from FN1-associated stromal remodeling in ulcerative colitis.” The archive includes analysis and figure-generation scripts, frozen and processed source tables used for...

Advancing the sterile insect technique for the New World screwworm fly: the case for CRISPR-engineered genetic sexing strains.

It is argued that establishing a screwworm GSS would provide immediate benefits to existing management programs and hope this synthesis will inform and encourage future studies on this effort.

Multiplex CRISPR/Cas12a editing of ROC5, GS3, GW2, and LARGE2 reveals effects on yield-related traits in rice cultivar Kasalath

'Epigenetic' editing is here: gene-tagging technique banishes hepatitis B virus.

Waterlogging and hypoxia in plant abiotic stress

Integrating CRISPR/Cas-based gene editing, genomic selection, multi-omics approaches, and improved agronomic practices offers promising avenues for developing flood-resilient crop varieties under changing climatic conditions.

Genomic insights into the mitochondrial architecture of Sarcandra glabra: structure, evolution, and functional implications

This work offers the first comprehensive analysis of the S. glabra mitochondrial genome, highlighting its structural complexity, dynamic RNA editing, signatures of adaptive evolution, and its phylogenetic position among basal angiosperms.

Molecular Targets and Trait Innovation in CRISPR-Edited Vegetable Crops: An Up-to-Date Review (2020–2026)

Vegetable crops are of fundamental nutritional and economic importance worldwide, yet their improvement through conventional breeding remains time consuming and constrained by genomic complexity. For that reason, over the period 2020–2026, CRISPR/Cas-mediated genome editing, a new genomic technique (NGT), has emerged a...

13. DIRECT CRISPR-EDITING OF RS4702 DECIPHERS CELL-TYPE SPECIFIC EPISTATIC INTERACTIONS WITH SCHIZOPHRENIA POLYGENIC RISK

Background Schizophrenia (SCZ) has a complex, heterogeneous, and highly polygenic genetic architecture. Polygenic risk scores (PRS) assume additive genetic effects and may not capture epistatic or gene-by-environment interactions. However, PRS analyzed in conjunction with functional genomic approaches using CRISPR edit...

Precision engineering of upstream open reading frames for translational regulation and crop improvement

Upstream open reading frames (uORFs) are pervasive cis-regulatory elements in the 5′untranslated regions (UTRs) of eukaryotic mRNAs that modulate translation of downstream main open reading frames (mORFs) by shaping ribosome scanning, initiation, and reinitiation. By operating downstream of transcription, uORFs enable...

From Association to Causality: Next-Generation GWAS, Pangenomes, and Multi-Omics Driving Gene Discovery and Precision Breeding in Cereal Crops

Advances in pangenomics, multi-omic integration, and gene-editing technologies are helping to overcome limitations in conventional GWASs by improving candidate-gene identification and functional validation, moving cereal genomics from statistical association toward biologically validated targets for developing more res...

Application of CRISPR–Cas9 technology in the treatment of chronic lymphocytic leukemia with mutant P53 gene

Aim This study proposes the implementation of clustered regularly interspaced short palindromic repeats (CRISPR)–CRISPR-associated protein 9 (Cas9) technology for gene therapy targeting genetic mutations of P53 gene in human lymphocytes affected by chronic lymphocytic leukemia, (CLL), offering new opportunities for eff...

Unintended omics changes in two gene-edited wheat lines exhibit less variation than in conventional lines and show no detectable adverse effects on the aphid Schizaphis graminum.

A key safety concern for gene-edited (GE) crops is whether unintended molecular changes associated with gene editing pose risks to non-target organisms (NTO). Here, we combined proteomic and metabolomic profiling with insect bioassays to assess the effects of two GE wheat lines (mildew resistance Locus O (MLO)- and Tri...