protein folding

Microsecond Barrier-Crossing Collapse in Cytochrome c Folding Revealed by Two-Dimensional Fluorescence Lifetime Correlation Spectroscopy

The results reveal that the collapse exhibits two-state dynamics between structurally distributed ensembles, occurring on a time scale of tens of microseconds across all variants, indicating a global contraction of the whole protein structure with a substantial free energy barrier.

Mitochondrial Genome Comparison and Phylogenetic Analysis of the Family Carabidae (Coleoptera: Adephaga)

These findings provide new mitochondrial genomic evidence for elucidating the evolutionary history of Carabidae and underscore the necessity of broader taxonomic sampling, as well as the integration of nuclear genomic data, to further resolve the contentious relationships within this diverse beetle family.

Salt Stress Accelerates Tyrosine Depletion by Enterococcus lactis KUST2812: Multi-Omics Insights into Metabolic Remodeling

E evaluation in BBP fermentation suggested that E. lactis KUST2812 may reduce tyrosine accumulation without causing a significant increase in tyramine, and coordinated responses involving the Na+/H+ antiporter system, compatible solute transporters, and oxidative stress markers contributed to the metabolic basis for sa...

Effect of encapsulation on hygroscopic, structural, physico-thermal and in vitro release properties of DPP-IV inhibitory peptides-rich sodium caseinate hydrolysates

Considering the increasing worldwide prevalence of Type 2 diabetes mellitus (T2DM), utilizing milk protein-derived bioactive peptides offers a promising and sustainable solution to manage hyperglycemia. However, formulating functional foods rich in Dipeptidyl peptidase-IV (DPP-IV) inhibitory peptides is hindered by the...

Transcriptomic and Genomic Insights into HSP-Mediated Protein Homeostasis in Ulmus parvifolia under Temperature Stress

CBFLivUni/CNOSerumProteinsPublic: CNO Serum Proteomics Biomarkers: peer review release, minor updates.

This repository contains the code and analysis workflows associated with the publication "Serum Proteome Profiling Reveals a Candidate Biomarker Signature for Paediatric Chronic Nonbacterial Osteomyelitis (CNO)/Chronic Recurrent Multifocal Osteomyelitis (CRMO)” by Eve Roberts, Euan McDonnell, Phillip Brownridge, James...

Simulation of Biomolecular Condensates with Martini3-IDP

Inside our cells, some proteins remain flexible and dynamic rather than folding into rigid shapes. These intrinsically disordered proteins (IDPs) can assemble into liquid-like biomolecular condensates, like oil droplets in water solution, to organize cellular biochemistry without encapsulating lipid membranes while con...

A Single-Administration AAV Gene Therapy Expressing an Affinity-Detuned Feline AIM (m-fAIM) for Chronic Kidney Disease in the Domestic Cat: A Theoretical Development Program

This work presents a theoretical development program for a single-administration adeno-associated virus (AAV) gene therapy designed to treat chronic kidney disease (CKD) in the domestic cat. The approach centers on the delivery of an affinity-detuned variant of feline Apoptosis Inhibitor of Macrophage (m-fAIM), enginee...

Processed transcriptomic and proteomic datasets supporting the multi-omics analysis in the manuscript

This dataset contains processed transcriptomic and proteomic data generated from inguinal white adipose tissue collected from control mice, obesity-associated PCOS model mice, and PCOS mice treated with high-dose Atractylodis Rhizoma-derived exosome-like nanovesicles (AR-ELNs). These data support the multi-omics analys...

Breaking timescales with generative sampling of conformational transitions

Gen-COMPAS is introduced, a generative committor-guided path-sampling framework that reconstructs rare biomolecular transition pathways and reveals the underlying thermodynamics and kinetics without using predefined collective variables or brute-force sampling, at an acceptable computational cost.

Exosome-Mediated Bidirectional Signaling Regulates Glioma Stem-Cell Homeostasis and Radiation-Induced Plasticity

Exosome-mediated intercellular communication is identified as a potential mechanism regulating GBM cellular homeostasis, treatment resistance, and recurrence and is used to establish a bidirectional feedback circuit between stem-like and differentiated tumor-cell compartments.

Co-translational O-glycosylation driven by GALNTs spatial reprogramming fosters pancreatic cancer growth

It is shown that GALA subverts the normal temporal sequence of folding and glycosylation in pancreatic ductal adenocarcinomas and in murine pancreatic tumours, where it stimulates growth.