Engineering mRNA stability is a promising yet underexplored approach for improving recombinant protein production in bacterial systems. In this study, we evaluated the effect of synthetic 3'-UTR hairpin structures on mRNA stability and protein yield in Escherichia coli using two SUMO-fusion expression systems. Hairpin...
Z. Khasanshina, M. Yarovikova, E. Buslaeva et al.· Protein Expression and Purif...· 0 citations
The ribosome is a universally conserved macromolecular machine responsible for protein synthesis across all domains of life. It is composed of two subunits, the small ribosomal subunit (SSU) and the large ribosomal subunit (LSU), which come together during translation to form the functional ribosome. In Saccharomyces c...
Rafał Piwowarczyk· Digital Commons - RU (Rockef...· 0 citations
Results identify B2043 as a negative regulator of biofilm formation acting predominantly during attachment, provide the first experimental evidence for c-di-GMP-dependent biofilm regulation in C. necator H16, and establish a functional-genomics framework — together with eleven further candidate loci — for engineering p...
Janek R. Weiler, C. J. Lapp, Johannes Gescher et al.· Biofilm· 0 citations
Abstract This sub-paper, "Fractal Compact Manifold Theory Core Mechanics" serves as a concise, standalone distillation of FCMT's foundational elements, emphasizing the atemporal symmetry breaking, field emergence, Lagrangian formulation, projection operator, and foliation map. By focusing solely on these core mechanics...
Hosack, Zachary· Zenodo (CERN European Organi...· 0 citations
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N-glycosylation plays essential roles in the folding, trafficking, and maturation of proteins in the secretory pathways, but how individual protein- and site- specific glycosylation rewires under endoplasmic reticulum (ER) stress is unknown. Particularly, intact glycopeptide data that retain the connectivity between gl...
Alexander Black, Tiffany Ngo, Pandi Boomathi Pandeswari et al.· Molecular & Cellular Proteom...· 0 citations
JULÄINE produced sustained, clinically meaningful improvements in patient-perceived aesthetic outcomes with high treatment satisfaction over 12 months, and the consistent perception of natural-looking results is in line with the progressive, collagen-building mechanism of PLLA-LASYNPRO.
A. Ribé, Ulrika Erhardt, Karina De Almeida Caramico et al.· Aesthetic surgery journal· 0 citations
Synthetic multi-species consortia provide valuable insights into the ecological and structural dynamics of complex microbial biofilms. However, the specialized functional contributions of individual components under severe nutrient limitations remain poorly understood. This study investigated the population dynamics, m...
T. Pankratov, A. Hakobjanyan· Ecologies· 0 citations
KinConfBench is introduced, a curated benchmark of 2225 high-quality human kinase chains to evaluate the ability of four state-of-the-art cofolding models—Boltz-2, Chai-1, Protenix, and RoseTTAFold-All-Atom—to recover both canonical and rare conformational states.
Kun-Yang Sun, T. Head-Gordon· npj Drug Discovery· 1 citation
We evaluated agricultural innovations such as push-pull (PP), vegetable-integrated push-pull (VIPP), and black soldier fly frass fertilizer (BSFFF) for their transformative potential in rural smallholder agriculture. Using 12 cropping systems comprising two crops (maize and cowpea), BSFFF and diversified systems (PP an...
E. Omuse, H. Machekano, D. Mutyambai et al.· npj Sustainable Agriculture· 0 citations
A putative diagnostic biomarker for CCM, YES1, was demonstrated to be significantly elevated in the plasma of affected patients, with high correlative expression in the source tissue, which further support the development of CCM-related noninvasive biomarkers and may add insight into their pathogenesis.
Yousef Hattar, J. Sesen, Tyra Martinez et al.· Journal of Neurosurgery: Ped...· 0 citations
NitroXAI v0.1.1 is an interpretable framework for residue-level S-nitrosylation (SNO) site prediction. The release provides two frozen ensemble predictors for identifying candidate S-nitrosylated cysteineresidues from complete protein sequences: • SNO-CLIM: a lightweight CNN-BiLSTM baseline using handcrafted biochemica...
Soumyadeep Ray, Ganesh Bagler· Zenodo (CERN European Organi...· 0 citations
Here we developed a DNA-centric strategy for optimizing site-specific recombination by rationally engineering chimeric attachment sites. The high-activity att variants enhance Bxb1-mediated integration efficiency in human cells and plants. Among these att variants, the engineered attB(V111) site achieved 51.9% integrat...
A new machine-learning framework aims to improve the success rate of computational protein design while moving away from results that reproduce sequences found in nature.
