protein folding

Sodium butyrate improves growth, antioxidant capacity and intestinal health in juvenile common carp fed high-plant-protein diets

ENGINEERING 3'-UTR HAIRPIN STRUCTURES TO MODULATE MRNA STABILITY AND RECOMBINANT PROTEIN PRODUCTION IN ESCHERICHIA COLI.

Engineering mRNA stability is a promising yet underexplored approach for improving recombinant protein production in bacterial systems. In this study, we evaluated the effect of synthetic 3'-UTR hairpin structures on mRNA stability and protein yield in Escherichia coli using two SUMO-fusion expression systems. Hairpin...

Principles of Co-Transcriptional Ribosome Assembly Nucleation and Quality Control

The ribosome is a universally conserved macromolecular machine responsible for protein synthesis across all domains of life. It is composed of two subunits, the small ribosomal subunit (SSU) and the large ribosomal subunit (LSU), which come together during translation to form the functional ribosome. In Saccharomyces c...

Identification of genetic determinants that promote biofilm growth under heterotrophic conditions in Cupriavidus necator using transposon enrichment

Results identify B2043 as a negative regulator of biofilm formation acting predominantly during attachment, provide the first experimental evidence for c-di-GMP-dependent biofilm regulation in C. necator H16, and establish a functional-genomics framework — together with eleven further candidate loci — for engineering p...

Fractal Compact Manifold Theory Core Mechanics: Multifractal Unification of Quantum Mechanics, Gravity, and Consciousness

Abstract This sub-paper, "Fractal Compact Manifold Theory Core Mechanics" serves as a concise, standalone distillation of FCMT's foundational elements, emphasizing the atemporal symmetry breaking, field emergence, Lagrangian formulation, projection operator, and foliation map. By focusing solely on these core mechanics...

Micro- and macro- heterogeneity of N-glycoproteome remodeling in endoplasmic reticulum stress

N-glycosylation plays essential roles in the folding, trafficking, and maturation of proteins in the secretory pathways, but how individual protein- and site- specific glycosylation rewires under endoplasmic reticulum (ER) stress is unknown. Particularly, intact glycopeptide data that retain the connectivity between gl...

Effectiveness, Patient Satisfaction, and Safety of a Next-generation PLLA Collagen Biostimulator for Nasolabial Fold Augmentation: 12-month Results of a Prospective Multicenter Study.

JULÄINE produced sustained, clinically meaningful improvements in patient-perceived aesthetic outcomes with high treatment satisfaction over 12 months, and the consistent perception of natural-looking results is in line with the progressive, collagen-building mechanism of PLLA-LASYNPRO.

Formation and Ecological Dynamics of Synthetic Biofilms Derived from Microbial Components of the Cladonia arbuscula Thallus

Synthetic multi-species consortia provide valuable insights into the ecological and structural dynamics of complex microbial biofilms. However, the specialized functional contributions of individual components under severe nutrient limitations remain poorly understood. This study investigated the population dynamics, m...

A curated benchmark for cofolding models on kinase conformational states

KinConfBench is introduced, a curated benchmark of 2225 high-quality human kinase chains to evaluate the ability of four state-of-the-art cofolding models—Boltz-2, Chai-1, Protenix, and RoseTTAFold-All-Atom—to recover both canonical and rare conformational states.

Coupling intensified push–pull technology with insect frass biofertilization improves land productivity, protein yield and carbon stocks

We evaluated agricultural innovations such as push-pull (PP), vegetable-integrated push-pull (VIPP), and black soldier fly frass fertilizer (BSFFF) for their transformative potential in rural smallholder agriculture. Using 12 cropping systems comprising two crops (maize and cowpea), BSFFF and diversified systems (PP an...

YES1, a plasma-based biomarker for cavernous malformations.

A putative diagnostic biomarker for CCM, YES1, was demonstrated to be significantly elevated in the plasma of affected patients, with high correlative expression in the source tissue, which further support the development of CCM-related noninvasive biomarkers and may add insight into their pathogenesis.

NitroXAI: An Interpretable Deep Learning Framework for Human S-Nitrosylation Site Prediction

NitroXAI v0.1.1 is an interpretable framework for residue-level S-nitrosylation (SNO) site prediction. The release provides two frozen ensemble predictors for identifying candidate S-nitrosylated cysteineresidues from complete protein sequences: • SNO-CLIM: a lightweight CNN-BiLSTM baseline using handcrafted biochemica...