Rab Interactions identified by DEEPN.

Abstract

Rab GTPase Y2H Interactome — Verified Hit DatasetThis dataset contains the curated set of protein-protein interactions identified in a yeast two-hybrid (Y2H) screen against 36 human Rab GTPases, each tested in both nucleotide-locked conformations: GTP-bound (constitutively active, Q→L mutant) and GDP-bound (dominant-negative, T→N mutant). The dataset comprises 591 confirmed interactions, each supported by an Interaction Data Package (IDP) verification workbook containing 5' and 3' junction-mapping plots, sequencing read-depth coverage, and DESeq2/Bayesian statistical support (enrichment, log2 fold-change, and significance values) for both the selected and non-selected yeast populations.Unified_Rab_Interaction_List.xlsx indexes all 591 hits with their RefSeq accession, estimated interacting fragment (amino acid span), and full statistics, hyperlinked to the corresponding IDP workbook. IDP files are organized into per-Rab, per-conformation subfolders (e.g. Rab19-GTP, Rab19-GDP).Prior to inclusion, candidate interactions were filtered for biological plausibility: vector-alone (bait-independent) background artifacts, and interactions mapping to the extracellular/ER-lumenal domain of an integral membrane protein or to a secreted protein, were excluded as topologically inconsistent with a genuine cytosolic two-hybrid interaction. Excluded candidates and the rationale for each exclusion are provided in TOTAL_NonSpecific_NonRelevant_LIST.xlsx.