The rules governing promoter competition are uncovered, its impact on tuning gene expression levels and genome evolution is highlighted, and it is suggested that transcriptional units producing transcripts of sufficient level and length can mediate insulation independently of CTCF and cohesin.
This Perspective discusses mechanisms enabling long-range enhancer–promoter communication and the potential advantages that distal enhancer positioning might confer on the establishment of developmental gene expression programs.
It is proposed that SNAI1/2 function as a molecular dimmer switch—modulating nucleosome dynamics to calibrate enhancer output to achieve precise spatiotemporal control of transcript dosage.
Lucia Ichino, Kaelan J. Brennan, Ben Mallory et al.· bioRxiv· 0 citations
The chromatin organization of the Drosophila shavenbaby locus is investigated to identify a robust, multi-factorial enhancer-promoter hub that is shaped by distinct regulatory inputs across developmental contexts.
Sujay Naik, Srijani Roy, Ella Preger-Ben Noon· bioRxiv· 0 citations
Findings establish COMPACTs as a practical alternative to native promoters for various applications, including cell therapies, gene therapies, and biomanufacturing.
Chaja Katzman, S. Matusevich, Shir-Liya Dadon et al.· bioRxiv· 0 citations
Abstract HOTAIR (HOX transcript antisense RNA) is a HOXC-cluster long intervening non-coding RNA (lincRNA) whose cancer relevance is tightly coupled to how its transcription is wired into hormone, hypoxia, inflammatory, and developmental signaling. HOTAIR is known to associate with cancer cell proliferation, motility,...
Aritra Gupta, Kartiki V. Desai· Biochemical Society Transact...· 0 citations
The three-dimensional organization of the genome enables enhancers and promoters to interact across vast distances and direct transcription. Yet whether architectural elements, such as insulators, serve as rigid, passive barriers or as dynamic, active organizers of this communication remains unclear. Here, using single...
