AI Networking Cookbook: Practical recipes for AI-assisted network automation and development
TL;DR
This work integrated the MutaT7 system, which uses two base deaminases, into phage-assisted evolution to direct all four transition mutations specifically to the GOI, promoting the accumulation of all transition mutations, providing greater diversification of the GOI.
This review will focus on three core strategies-traditional random evolution, global accelerated evolution, and targeted continuous evolution-systematically elucidating their developmental trajectories, applications in the construction of microbial cell factories, and future directions.
Targeted mutagenesis is a crucial tool for in vivo continuous evolution. However, existing methods for long-range targeted mutagenesis in vivo often face limitations such as low efficiency, poor host compatibility, operational complexity, and uneven distribution of mutations across these windows. In this study, we deve...
ABSTRACT To overcome the labor‐intensive nature of traditional directed evolution, the promoted Escherichia coli‐assisted continuous evolution (PEACE) system has been developed. PEACE 1.0 employs a cytidine deaminase‑T7 RNA polymerase (T7 RNAP) fusion for targeted mutagenesis and integrates a toxin–antitoxin selection...
Plasmids are key drivers of bacterial adaptation, yet the mechanisms that generate their diversity remain poorly understood. Here, we show that mobile genetic elements (MGEs) orchestrate a fusion-deletion life cycle that repeatedly remodels plasmids in Staphylococcus aureus . Large-scale genomic analyses reveal t...
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