Discovery of novel membrane-associated calpains in Euglena gracilis: molecular insights into proteolysis in an ecologically important protist

Enzymatic formation of a conserved isoaspartate in ribosomal protein uS11

Isoaspartate (isoAsp) formation is typically viewed as a “molecular clock” through nonenzymatic degradation of aspartate or asparagine during protein aging. Here we report a nearly universal enzymatic pathway for the formation of a conserved isoAsp in the bacterial ribosomal protein uS11. Proteome-wide protein-protein...

Structural insights into a putative BLF1-like deamidase from Methylomonas sp. Kb3.

Structural features suggest that the Methylomonas protein that is identified may act as a BLF1-like deamidase but against a currently unknown target, and that other bacterial proteins with similar sequence fingerprints could play important roles in broader bacterial biology.

An In Silico Investigation of the Structural and Evolutionary Features of MAPEG Proteins in Bacteria

The Membrane-Associated Proteins in Eicosanoid and Glutathione metabolism (MAPEG) superfamily comprises integral membrane proteins involved in lipid metabolism, detoxification, and redox regulation. While extensively characterised in eukaryotes, bacterial MAPEG homologs remain poorly understood. In this study, we condu...

A high-confidence inventory of the proteins in the mitochondrion-related organelle of Giardia

SUMMARY Giardia lamblia is a microaerophilic protozoan parasite that infects nearly 280 million people worldwide each year. Giardia lacks a mitochondrial genome and mitochondrial enzymes for oxidative phosphorylation. However, it retains mitochondrion-related organelles (MROs) that house an iron-sulfur cluster (ISC) as...

Analysis of Phycodnaviridae Tetrapyrrole Enzymes: Subcellular Localization and Functional Divergence from Putative Host Homologs

In silico characterization of SAP55: insights into a predicted phytoplasmal M41-like metallopeptidase effector with potential eukaryotic host dual lipidation motifs

This study proposes a working model for SAP55-mediated host manipulation, and establishes a foundation for future experimental investigation, as well as providing a comprehensive computational framework for a candidate membrane-associated candidate effector.