This nMS-based workflow offers a broadly applicable framework for resolving the catalytic states and inter-site communication of oligomeric enzymes that are otherwise difficult to uncover by conventional structural methods.
The crystal structure of Petunia hybrida BS is reported, which reveals an α2β2 heterotetrameric arrangement, establishing BS as a rare heterotetrameric plant SDR and demonstrating how subunit specialization and intersubunit arrangement enable function, providing principles for understanding and engineering multimeric e...
Jason O. Matos, Jihee Lee, Ramasamy P. Kumar et al.· Science Advances· 0 citations
This work presents a thorough structural investigation that yielded the first ligand-bound structure of this protein family, which required the generation of artifact-free apo crystals, and developed a novel, low-consumption, robust kinetic assay.
Brent Runge, Hande Oktay, Ian J. Fucci et al.· Journal of Structural Biolog...· 0 citations
The molecular basis for substrate selectivity in a deubiquitinating enzyme is described, an unexplored area in the enzymes that counteract ubiquitin E3 ligases, an unexplored area in the enzymes that counteract ubiquitin E3 ligases.
Rishi S. Patel, Nipuni M. Pannala, Chih-Hsuan Lai et al.· bioRxiv· 0 citations
It is demonstrated that the availability of the RuvC catalytic channel is determined not by induced fit binding but by the chemical cleavability of the occupant, which provides a theoretical framework and additional insights for developing more precise gene-editing tools and designing Cas12a-based in vitro diagnostic p...
The specificity and catalytic efficiency of enzymes make them attractive for applications ranging from therapeutics to chemical manufacturing. However, it remains challenging to identify specific structural and dynamic mechanisms by which enzymes achieve their catalytic rate enhancements as well as to re-engineer enzym...
Elijah Karvelis, Bruce Tidor· Journal of Physical Chemistr...· 0 citations
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