3D co-culture model of tumor spheroid and endothelial cells unveils ccRCC aberrant vasculature and distinct sensitivity to targeted treatments

Hypoxia-activated endothelial cells drive stemness and vasculogenic mimicry in NSCLC via a HIF-1α/ITPR3 axis.

Hypoxia-activated endothelial cells drive NSCLC aggressiveness and VM through a paracrine axis involving ITPR3-mediated ER calcium activation, and this HIF-1α/ITPR3 axis represents a potential therapeutic target for disrupting the tumor-vascular niche in NSCLC.

Cellular adhesion-dependent 3D morphogenesis indicating brain tumor aggressiveness and chemosensitivity in spherical cavity culture

Malignancies of the nervous system remain a critical challenge in oncology, contributing substantially to cancer-related mortality in children and adults. Their aggressive behavior is influenced by adhesion heterogeneity of the tumor cells, as distinct patterns of cell-to-cell and cell-to-matrix interactions shape tumo...

Micro-vascularized Multicellular Pancreatic Ductal Adenocarcinoma (PDAC) spheroid: A novel 3D PDAC Research Model.

This model enables analysis of tumor-endothelial-stromal interactions in a pancreas-derived dECM environment and supported PDAC spheroid formation and the CD44-associated endothelial phenotype.

A 3D Tumor Spheroid Platform to Model Fusobacterium nucleatum-Induced Tumorigenesis.

A detailed protocol is presented in which colorectal cancer cells are seeded into ultralow attachment plates and co-cultured with F. nucleatum either prior to spheroid formation, to model early tumor development, or after spheroids have been established, to model tumor progression.

Angiogenesis inhibition in high grade glioma

Bidirectional phenotypic transition in macrophages influences triple-negative breast cancer cell immune evasive patterns and response to chemotherapies in a co-culture spheroid model

An effective 3D co-culture system of breast cancer cells and integrated macrophages for studying dynamic cellular phenotype changes and reciprocal interactions in a heterogeneous environment to mimic aspects of the TME and enhance the accuracy of preclinical in vitro treatment response studies is presented.