These findings identify nucleotide class composition as a previously unrecognized parameter governing IVT-mRNA function and establish hybrid ribonucleotide-deoxyribonucleotide backbone engineering as a versatile strategy to expand the chemical space for next-generation mRNA therapeutics.
Replicative DNA polymerases are responsible for the high-fidelity duplication of genomes in all organisms. The overall DNA replication process is highly accurate, with deoxyribonucleoside monophosphate (dNMP) errors occurring on the order of 1 in tens of millions of replicated bases. However, DNA polymerases are more p...
Julianna R. Cresti, Lyle A. Simmons· Critical reviews in biochemi...· 0 citations
The “RNA World” hypothesis posits a reasonable scenario for the origin of life. A question central to this scenario is: which RNA species may have emerged as the first ribozyme, thereby initiating the earliest stage of life? To date, experimental and computational studies have focused on ribozymes playing a constructiv...
A novel riboswitch platform based on RNA triplexes stabilizing non-polyadenylated transcripts while still allowing translation of the respective messenger RNA is presented, designed to function independently of nuclear processes such as transcription and RNA processing.
Pierre Gommeringer, Elisabeth Müller, S. Köse et al.· Nucleic Acids Research· 1 citation
circDesign is presented, an algorithm that explicitly incorporates IRES structural deviation into circRNA sequence design while jointly optimizing codon adaptation and thermodynamic stability and establishes IRES structural preservation as a mechanistic design principle for circRNA engineering and position circDesign a...
Congcong Xu, F. Jiang, Yifan Jiang et al.· Angewandte Chemie· 0 citations
Viruses encode diverse regulatory elements, but their breadth and mechanisms remain poorly defined. To address this gap, we performed massively parallel reporter assays spanning ∼200,000 genomic segments from 297 vertebrate-infecting viral genera. We identified numerous viral elements that enhance RNA stability and tra...
Jenny J Seo, Che-Min Lee, Dongbin Lim et al.· Cell· 1 citation
ADP-ribosylation (ADPr), long recognized as a canonical protein post-translational modification, has recently expanded to include targeting nucleic acids, uncovering a diverse landscape of noncanonical biological functions. Emerging evidence suggests that ADPr at the 5'-phosphate terminus of DNA is implicated in the DN...
Lingxiao Liu, Yang Lu, Zongxing Yu et al.· Journal of the American Chem...· 0 citations
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