Oct 2026· Advances in Biochemical Engineering/Biotechnology· 0 citations
Medicine
TL;DR
This chapter provides a comprehensive overview of major strategies in protein design including directed evolution, rational design, and semi-rational design, and emphasis is placed on recent advances in high-throughput screening technologies, identification of functionally critical residues, and optimization of site-specific mutations.
Protein engineering is a rapidly developing field of biotechnology that involves the modification or redesign of proteins to obtain improved, altered, or novel properties. The ability to engineer proteins has significantly expanded their applications in industrial biotechnology, medicine, agriculture, food technology,...
Vasudevan Ranganathan, M. Padma, D. Haripriya· Asian Journal of Biochemistr...· 0 citations
Enzymes are efficient and selective biocatalysts, yet obtaining enzymes with desired properties remains challenging due to vast sequence spaces, incomplete functional annotation, limited structural information, and costly experimental screening. Machine learning (ML) offers a powerful complement to address these limi...
Yeasts serve as premier eukaryotic hosts for microbial cell factories, enabling the production of recombinant proteins, biofuels, and high-value natural products. Precise transcriptional control is paramount for balancing complex metabolic pathways and maximizing target product yields. Promoters, as the key regulatory...
Zhen-Zhen Bai, Tian Jiao, F. Darvishi et al.· ACS Synthetic Biology· 0 citations
Key genetic engineering approaches, including CRISPR-based genome editing, transcriptional and translational regulation, and modular DNA assembly, and modular DNA assembly are discussed together with strategies for metabolic pathway design, enzyme engineering, flux balancing, and dynamic regulation.
Ji-Peng Jiang, Xin Zhang, Li-Xin Zhang et al.· Advances in Biochemical Engi...· 0 citations
The integration of artificial intelligence with protein engineering has created unprecedented opportunities for enzyme discovery and the enhancement of overall catalytic performance. In this study, a novel nitrilase, BhNIT, was identified from Bradyrhizobiumhuanghuaihaiense through a computationally assisted screenin...
Directed evolution (DE) represents a pivotal interdisciplinary paradigm that synergistically integrates biotechnology, computational methodologies, and precision analytical instrumentation. It enables accelerated, controllable in vitro laboratory evolution of diverse biomolecules toward predefined functional targets. T...
Li-Hua Liu, Yu Zhang, Yu-Cheng Hu et al.· Biotechnology Advances· 0 citations
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