Aug 2026· Insects· Vol 17, pp. 836· 0 citations· 63 references
Medicine
TL;DR
Homology modeling and molecular docking revealed that CYP9A58 and CYP9A59 can form stable complexes with both insecticides, showing notably stronger binding affinity for lambda-cyhalothrin.
Abstract
Simple Summary Cytochrome P450s are crucial for insecticide detoxification, yet the molecular mechanism underlying P450-mediated pyrethroid detoxification in S. frugiperda remains largely unclear. Enzyme activity assays suggested the involvement of P450s in pyrethroid detoxification, with CYP9A58 and CYP9A59 being significantly induced by bifenthrin and lambda-cyhalothrin. RNAi-mediated knockdown of these genes significantly increased larval mortality upon lambda-cyhalothrin exposure. Homology modeling and molecular docking revealed that CYP9A58 and CYP9A59 can form stable complexes with both insecticides, showing notably stronger binding affinity for lambda-cyhalothrin. These findings enhance our understanding of P450 genes in insecticide stress responses and offer valuable insights into S. frugiperda control.
Findings provide direct biochemical evidence that CYP4C64 contributes to nitenpyram detoxification in B. tabaci and demonstrate that the Bac-to-Bac expression system is an effective platform for functional characterization of insect P450 enzymes involved in insecticide metabolism.
Wenhan Cheng, Han-Tang Lu, Chengjia Zhang et al.· Journal of Agricultural and...· 0 citations
The cytochrome P450 monooxygenase system is a key detoxification pathway involved in insecticide metabolism in insects and plays a central role in the evolution of insecticide resistance. NADPH-cytochrome P450 reductase (CPR), a crucial electron transfer partner in cytochrome P450-mediated detoxification of xenobiotics...
Huijuan Yang, Jun-Yu Fu, Zhi-Wen Yi et al.· Parasites & Vectors· 0 citations
Simple Summary The oriental fruit fly is a serious quarantine pest of fruits and vegetables, and insecticides remain important for its management. Broflanilide is a relatively new insecticide, but little is known about how this pest responds to this compound. We compared gene expression in the midgut and fat body of fl...
Bo Liu, Si-Yi Chen, Zhi-Hong Li et al.· Insects· 0 citations
It is demonstrated that even resistance-associated detoxification genes that are considered functionally validated in vitro, can have strong context-dependent phenotypic effects in vivo, underscoring the complexity of resistance gene networks and highlighting the need to interpret CRISPR/Cas9 knockout phenotypes within...
S. Naessens, S. De Rouck, Marilou Vandenhole et al.· Insect Biochemistry and Mole...· 0 citations
Cytochrome P450 monooxygenases (P450s) play a pivotal role in metabolic herbicide resistance, yet demonstrating that a P450 can metabolize a herbicide is not sufficient to establish that it is responsible for resistance in weeds. The rapid expansion of transcriptomic resources and functional assays has accelerated the...
Satoshi Iwakami, Takuya Yamaguchi· Pest Management Science· 0 citations
BACKGROUND
Cowpea is a vital vegetable crop in tropical regions, but the Megalurothrips usitatus causes severe yield losses. Emamectin benzoate is widely used for control; however, resistance has emerged and the molecular basis remains unclear.
RESULTS
This investigation demonstrates through bioassay analysis that fi...
Wen-Bo Dong, Tian-Bao Huang, Wen-Jin Liu et al.· Pest Management Science· 0 citations
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