This study analyzed specific residues within the C-terminal α11 helix that are critical for stable association of Orc6 with the ORC complex in Drosophila and proposed that this NLS drives human protein to the nucleus and compensates for weaker Orc6-ORC(1-5) interactions by increasing the nuclear concentration of Orc6 and shifting the equilibrium toward formation of the fully assembled ORC complex at the DNA.
The results reveal a new C-terminus-mediated RPA-RecQ interaction in L. donovani that resembles the prokaryotic SSB-RecQ interaction mechanism, while maintaining functional conservation of eukaryotic DNA metabolism.
Mayukh Chakraborty, Susmita Ghosh, Surasree Chakraborty et al.· The FEBS Journal· 0 citations
The results define the dynamic architecture of the Rixosome and provide a structural framework for ITS2 processing during nuclear maturation of the eukaryotic 60S ribosomal subunit.
M. Thoms, Jing-Yi Zhu, Xia-Ying Hong et al.· bioRxiv· 0 citations
RNA editing in mitochondria is vital for many eukaryotes. In plants, mitochondrial C-to-U deamination editing is catalyzed by tens to hundreds of PPR-DYW proteins, each typically dedicated to a specific site. Where retained, PPR-DYW family expanded independently in all eukaryotic groups examined here—except in marine m...
M. Valach, L. Aguilar, Ninaad Kalla et al.· bioRxiv· 0 citations
Where recombination takes place is crucial as it determines the position of genetic reshuffling, which facilitates species evolution and adaptation. In many vertebrates, PRDM9 determines the location of double-strand breaks that initiates meiotic recombination. However, this only applies for Prdm9 orthologs that posses...
Amélie Rudler, Laurent Duret, C. Grunau et al.· bioRxiv· 0 citations
Set1 is the catalytic subunit of SET1C or COMPASS, which methylates histone H3K4 and serves as a scaffold for the association of seven tightly bound polypeptides. We have employed yeast two-hybrid screenings to determine the interactome of Set1 and each subunit, providing a unique resource for exploring known and novel...
P. Luciano, Kihyun Park, S. Audebert et al.· eLife· 0 citations
Chromosomes fold into distinct domains that regulate transcription, replication and repair. In addition to well-characterized topologically associating domains (TADs) and compartments, the diversity of heterochromatin domains remains poorly defined at the sequence level. Here, using single-molecule tracing of nascent h...
Dania Camila Pulido Barrera, J. E. You, Angelo L. Angonezi et al.· Nature Structural & Molecula...· 0 citations
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