gene editing

Targeting Covalently Closed Circular DNA (cccDNA): Emerging Therapeutic Strategies Toward a Functional Cure for Chronic Hepatitis B

Chronic HBV won't be resolved in the immediate future. Almost 300 million individuals live with it [1], as well as it causes roughly 820,000 deaths annually [1]—mainly due to conditions such as cirrhosis, liver failure, or liver cancer. The usual therapies, such as nucleos(t)ide analogues and pegylated interferon-alpha...

Diplonemid PPR-DYW protein involved in multisite RNA editing

RNA editing in mitochondria is vital for many eukaryotes. In plants, mitochondrial C-to-U deamination editing is catalyzed by tens to hundreds of PPR-DYW proteins, each typically dedicated to a specific site. Where retained, PPR-DYW family expanded independently in all eukaryotic groups. Marine microeukaryotes diplonem...

Genomic Mechanisms of Plant Adaptation to Salinity and Drought Stress: Genes, Networks, and Evolutionary Implications

This review brings together recent advances in identifying genes, regulatory networks, and evolutionary strategies underlying plant responses to osmotic stress and examines natural variation in stress tolerance, highlighting genomic signatures of selection in halophytes and xerophytes that provide insights for crop imp...

Diplonemid PPR-DYW protein involved in multisite RNA editing

RNA editing in mitochondria is vital for many eukaryotes. In plants, mitochondrial C-to-U deamination editing is catalyzed by tens to hundreds of PPR-DYW proteins, each typically dedicated to a specific site. Where retained, PPR-DYW family expanded independently in all eukaryotic groups. Marine microeukaryotes diplonem...

Biomimetic Cell Membrane-Coated Nanocarriers for CRISPR-Cas9 Delivery: Recent Advances, Challenges and Future Perspectives

The CRISPR-Cas9 system revolutionized genome editing and is a very promising platform for the treatment of cancers, genetic disorders, and infections. Yet the clinical translation of CRISPR-Cas9 is hampered by some major bottlenecks related to delivery issues, such as fast degradation, poor cellular uptake, and immunog...

TCF4 at the crest of development: a zebrafish model to explore craniofacial and gastrointestinal defects in Pitt-Hopkins syndrome

Light is shed on TCF4 as a key regulator of neural crest-derived lineages that contribute to craniofacial and Enteric Nervous System development and a new perspective on two major clinical features of PTHS is provided.

Precision Medicine in Viral Hepatitis: Current Advances, Challenges and Future Perspectives

Viral hepatitis is a major global health problem, being the leading cause of chronic liver disease, cirrhosis, and hepatocellular carcinoma. The World Health Organization (WHO) has reported that hepatitis B and C viruses cause 1.34 million deaths yearly, and despite the availability of effective vaccines and treatments...

Deciphering the role of APOBEC3B in t(4;14) multiple myeloma

Multiple myeloma (MM) is a genetically heterogeneous plasma cell malignancy characterized by recurrent chromosomal abnormalities and progressive genomic instability. Among these, the t(4;14)(p16;q32) defines a high-risk molecular subtype associated with poor clinical outcomes despite advances in therapy. This transloca...

Rational engineering of high-fidelity base editors for therapeutic gene editing

The full abstract for this thesis is available in the body of the thesis, and will be available when the embargo expires.

Rewilding gut microbiomes of livestock: Evidence, approaches, and prospects for sustainable animal husbandry

If risks are rigorously managed, gut microbiota rewilding offers an evolution-informed path with the potential to support more sustainable animal production and is proposed to move rewilding from concept toward safe, scalable application.

CRISPR/Cas9-Mediated Dual Editing of BcSGR1 and BcSGR2 Exerts Additive Enhancement on Delaying Leaf Senescence in Non-Heading Chinese Cabbage

It is demonstrated that dual editing of BcSGR1 and BcSGR2 exerts an additive effect on delaying leaf senescence and enhances postharvest shelf life without incurring significant yield penalties under the experimental conditions.

Rice immunity against Asian rice gall midge: from resistance genes to precision breeding