gene editing

A novel needleless delivery of Cas9 ribonucleoprotein complex in Bemisia tabaci embryos for gene editing

This study overcomes a key bottleneck in CRISPR/Cas delivery to small insect embryos, opening new avenues for rapid, high-throughput, and cost-effective RNP delivery methods in insect embryos.

Inflammaging and immunosenescence-driven remodeling of the immune microenvironment in osteoarthritis: mechanisms, immune regulation and immune reprogramming

It is highlighted that future OA treatment may gradually shift from symptom-oriented management toward disease-modifying therapy based on the identification of immune endotypes, and further studies are required to validate the underlying molecular mechanisms, evaluate safety, and conduct clinical trials, thereby facili...

The Hidden Layer of MicroRNA Regulation in Gynecologic Cancers: IsomiRs, Arm Switching, and RNA Epitranscriptomic Modifications

This review explores how isomiR generation, miRNA strand selection, arm switching, and epitranscriptomic regulation expand the functional diversity of miRNAs in gynecologic cancers.

Applications of DNA Hydrogels in Osteoporotic Bone Defects

Pure and hybrid DNA hydrogels are proposed as novel therapeutic platforms to restore the dynamic balance between bone resorption and formation, thereby enhancing osteogenesis and facilitating bone regeneration and remodeling under osteoporotic conditions.

An upstream open reading frame represses translation of the neuronal potassium channel KCNQ2.

A single uORF in KCNQ2 is identified that is highly repressive of protein translation and it is demonstrated that mutations disabling the uORF start codon enhance synthesis of encoded potassium channels and weaken ribosome engagement at the uORF.

Applications of synthetic biology in biomedicine

This review systematically summarizes progress in foundational tools and key supporting technologies of synthetic biology, highlights innovative strategies and clinical value in biosensors, cell therapy, living therapeutics, and smart biomaterials, and provides an in-depth comparison of different chassis cells, deliver...

Future Directions in Pepper Breeding: Perspectives from the Pepper Breeding Panel at the 27th International Pepper Conference

Pepper ( Capsicum spp.) breeding is undergoing rapid change driven by climate change, evolving pest and disease pressures, specialized markets, and advances in breeding technology. To examine priorities for the next generation of pepper improvement, a panel session was organized during the 27th International Pepper...

Enhancing genetic gain in major cereal crops using speed breeding, genomic selection and gene editing technologies

Unlocking non-model organisms with CRISPR-Cas: A roadmap for sustainable biotechnology.

It is concluded that bridging the gap between foundational CRISPR research and its real-world applications is imperative and future efforts should focus on democratizing tools via open-source platforms, advancing delivery systems, and fostering sustainable innovation through synthetic biology integration to fully reali...

Application of Human Genome Editing Technologies in the Law of Certain States

This article is devoted to the legal regulation of the use of genomic editing technology in individual states. In general, it can be stated that in most legal systems there are restrictions on genomic editing in relation to hereditary genes, however, in rare cases exceptions are allowed, which must be recognized as jus...