gene editing

Jasmonate biosynthesis regulates key events in barley vegetative and reproductive development.

Cereal grain is a global food staple. Filial tissues in grain, such as the embryo and endosperm, grow embedded in the maternal ovule and surrounding ovary wall. Maternal tissues provide nutrition and protection but are progressively reduced and degraded as filial tissues expand, driving grain expansion between floret h...

Moniemäksinen geeniterapia lentiviraalisesti toimitetuilla Cas9(D10A):lla ja HDR-tehostajilla

The accumulation of mutations is the primary generator of hereditary diseases as well as ostensibly much of the symptomatology of aging. Various methods for the therapeutic repair of mutations have been devised, but most have focused on the repair of known hereditary diseases of fixed location, with none having demonst...

The Exploration of High-Performance Reaction Conditions on Trans-Cleavage Activity of Cas12a

Current advances and future directions of CRISPR/Cas9 screening for therapeutic target discovery in breast cancer

Breast cancer continues to be a major contributor to global cancer mortality, driven by pronounced molecular diversity and the frequent emergence of drug resistance, which together highlight the pressing demand for innovative treatment approaches. This review offers a thorough appraisal of CRISPR-Cas9-based functional...

Nanomaterial-assisted epigenetic engineering of the blood–brain barrier: A dual strategy for drug delivery and permeability control

Recent advances at the interface of nanotechnology, epigenetics, and neurobiology have created new opportunities to address the blood–brain barrier (BBB), a major challenge in the treatment of central nervous system disorders. Two complementary strategies are gaining increasing attention: the epigenetic modulation of B...

Potency, off-target analysis, and comparability in vivo gene-edited therapy development

The guide RNA is a drug substance or a critical component with its own manufacturing requirement. It is not just a reagent and should not be treated as such. Abi Pinchbeck, Commissioning Editor, Cell & Gene Therapy Insights, speaks with Kok-Seong Lim, Principal, Eight Crest Strategies, about the analytical characteriza...

Editorial: Europeans and xenotransplantation

Recent developments in the field of xenotransplantation have attracted considerable attention and constitute a major turning point for this scientific discipline. These recent investigations illustrate the major progress that has taken place in the last decade. In particular, advances in genetic-engineering techniques...

CRISPR/LbCas12a-Mediated Multiplex and Transgene-Free Genome Editing in Grapevine.

Agrobacterium ‐mediated transformation followed by regeneration without antibiotics‐dependent selection offers a a straightforward and effective approach for generating transgene‐free edited plants in grapevine, and the use of CRISPR/LbCas12a enables the simultaneous editing of multiple genes, thereby facilitating...

Comparative organelle genome analysis of three medicinal species of the genus Woodwardia (Blechnaceae): new insights into the phylogeny and molecular identification

Plants of the genus Woodwardia have a long history of medicinal use in China. Among these plants, Woodwardia japonica, Woodwardia unigemmata , and Woodwardia prolifera are the primary source species of Guan Zhong, a traditional Chinese medicine. However, because of morphological similarity among their dried...

Advances and Applications of Genome Editing in Precision Crop Breeding

Genome editing is becoming an important tool for crop improvement by enabling precise modification of genetic variation. CRISPR-Cas technologies have expanded beyond nuclease-induced mutations to include base and prime editing, transcriptional and epigenetic regulation, multiplex editing, organelle editing and targeted...

Harnessing Molecular Markers for Developing Climate-smart Rice Varieties

Rice production is increasingly constrained by climate-associated abiotic and biotic stresses, while conventional breeding is limited by dependence on phenotypic selection and lengthy breeding cycles. Climate-smart rice breeding seeks to develop high-yielding, resilient varieties with reduced environmental impact by in...

A genome-wide CRISPR knockout screen identifies host dependency factors and potential viral-induced cell death regulators for porcine deltacoronavirus infection

Porcine deltacoronavirus (PDCoV) is a globally circulating coronavirus of swine that causes severe enteric disease and mortality in neonatal piglets. Its broad host range and zoonotic potential raises concerns about continued spillover into human populations. However, the host factors that govern PDCoV infection remain...