gene editing

Transcriptomic Reanalysis Reveals Complement, Circadian, and Toll-Like Receptor Signaling Remodeling in Lactiplantibacillus plantarum-Induced Innate Immune Memory in Human Monocytes

Findings point to a multi-layered adaptive response where L. plantarum not only dampens canonical inflammatory signaling but also actively remodels cellular processes related to pathogen recognition, metabolic sensing, and stress responses.

101. Assessment of Published Bovine Guide RNA Design Practices.

CRISPR/Cas9 is the most widely applied gene-editing (GnEd) technology in livestock, with more than 200 published studies reporting the generation of live, gene-edited animals for agricultural applications. This system consists of a Cas9 nuclease that cleaves DNA and a 19-20 base pair guide RNA (gRNA) that directs the...

2.72P Optimization of intramuscular administration and in-vivo monitoring of gene-editing therapy in Duchenne muscular dystrophy

bactiviz: A Lightweight Command-Line Tool for Linear Collinearity Visualization of Bacterial Genomes

Comparative visualization of bacterial genome structure is an important component of microbial genomics, enabling the inspection of genome conservation, rearrangements, inversions, insertions and deletions, and large-scale differences in gene organization. I present bactiviz, a lightweight command-line tool for generat...

Super-Optimized Transgenic Synthetic Gyno-Genome (SOSG-2026): A Cross-Species Synthetic DNA Blueprint and Transgenic Sperm-Mediated Gene Transfer (SMGT) Methodology

वैज्ञानिक आविष्कार: सुपर-ऑप्टिमाइज्ड ट्रांसजेनिक सिंथेटिक गाइनो-जीनोम (Super-Optimized Transgenic Synthetic Gyno-Genome) मुख्य आविष्कारकर्ता (Principal Inventor): बंटी कुमावत (Banti Kumawat) विषय: सिंथेटिक बायोलॉजी, जेनेटिक इंजीनियरिंग और क्रिसपर-मल्टीप्लेक्सिंग (CRISPR Multiplexing) उद्देश्य: अत्यधिक दूध उत्पादन, जलवा...

Super-Optimized Transgenic Synthetic Gyno-Genome (SOSG-2026): A Cross-Species Synthetic DNA Blueprint and Transgenic Sperm-Mediated Gene Transfer (SMGT) Methodology

वैज्ञानिक आविष्कार: सुपर-ऑप्टिमाइज्ड ट्रांसजेनिक सिंथेटिक गाइनो-जीनोम (Super-Optimized Transgenic Synthetic Gyno-Genome) मुख्य आविष्कारकर्ता (Principal Inventor): बंटी कुमावत (Banti Kumawat) विषय: सिंथेटिक बायोलॉजी, जेनेटिक इंजीनियरिंग और क्रिसपर-मल्टीप्लेक्सिंग (CRISPR Multiplexing) उद्देश्य: अत्यधिक दूध उत्पादन, जलवा...

Protocol for quantification of AAV-CRISPR-mediated hepatic gene editing in mice.

This protocol enables quantification of INDELs and DNA-SVs, allowing for validation of gene disruption, and describes guide RNA (gRNA) design, plasmid cloning, delivery of AAV-CRISPR to the liver, and execution of liver DNA-editing analysis.

aomlomics/tourmaline: v2.2.0

Tourmaline v2.2.0 This release adds the tax-credit step: a fourth, optional workflow step that benchmarks reference databases and taxonomic classifiers, based on code from Bokulich et al. 2018. Bokulich NA, Kaehler BD, Rideout JR, Dillon M, Bolyen E, Knight R, Huttley GA, Caporaso JG. Optimizing taxonomic classificatio...

139. Award Talk: Integrative Physiology and Functional Genomics Reveal Novel Mechanisms Regulating Pubertal Development in Gilts.

Age at puberty is the earliest indicator of future reproductive potential of a gilt and because it is moderately heritable, identification of genomic markers can support marker-assisted selection to improve gilt development.

Ethical decisions under uncertainty? Guidance for initiating first-in-human trials involving genome editing

A three-tiered conceptual framework that operationalizes ethical principles to provide researchers with guidance on whether to initiate first-in-human studies involving gene transfer or genome editing is presented.

PS11-14. Performance and Physiological Responses of Beef Calves Carrying the Slick PRLR Allele During the Post-weaning Period.

Natural mutations in the prolactin receptor gene (PRLR) result in slick hair phenotype and improved thermoregulation. Gene-editing technologies allow the introduction of novel variants into PRLR, providing a strategy to rapidly incorporate slick phenotype into cattle populations. The objective of this study was to ev...

Responses of tomato to concurrent abiotic stresses, high temperature and drought: A review

Tomato production is increasingly threatened by the growing occurrence and severity of combined drought and high-temperature stress. Although the individual effects of high temperature and drought stress on tomato growth, physiology, and productivity have been widely investigated, their combined occurrence exerts more...