gene editing

Russel88/CRISPRCasTyper: v1.9.0

Python-native pipeline: CRISPR detection, HMM searches, and ORF prediction now run in-process (diced, pyhmmer, pyrodigal-gv); database/models ship with the wheel/sdist. Native repeat search backend: bundled edit-distance search (myers-batch + edlib) as a drop-in for BLAST+ near cas operons. --repeat_search_backend defa...

Genetic and molecular basis of gametophytic self-incompatibility in apple (Malus × domestica Borkh.): S-allele diversity, pollinizer selection, and prospects for cultivar improvement

FigureLab: a browser-based tool for assembling publication-quality scientific figures

FigureLab is a single-file, browser-based application for assembling publication-quality scientific figures from image data (microscopy, western blots, histology, gels, and clinical or specimen photographs). It runs entirely client-side with no installation, server, or internet connection required, so no image data eve...

The longevity gene APOE2 enhances pericyte function and reduces lipid droplets.

It is found that APOE2 expression supports pericyte functionality through increased lipid processing, providing mechanistic insight into the cerebrovascular protective effects of APOE2 in pericytes.

Overcoming delivery barriers of large all-in-one CRISPR/Cas9 plasmids using lysine-based lipid nanoparticles

Introduction Lipid nanoparticles (LNPs) are widely used as drug delivery systems (DDS) for the intracellular delivery of gene-editing tools such as the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) system. Previously, a lysine-headgroup cationic lipid (K3C16) was...

Advances in Wheat (Triticum aestivum L.) Genetics, Genomics and Breeding: Current Status, Tools and Future Perspectives for Sustainable Food Security

Wheat ( Triticum aestivum L.) is one of the most important cereal crops in the world. It supplies nearly 18–20% of global dietary calories and protein, sustaining the food security of over 2.5 billion people. Global food demand is projected to increase by 35–56% by 2050, placing additional pressure on wheat product...

CRISPR/Cas9-mediated generation of a green hypocotyl-marked male-sterile line for hybrid seed production in Solanum lycopersicum

Tomato ( Solanum lycopersicum ) is a self‑pollinating crop that exhibits significant heterosis. While F1 hybrid seeds offer high uniformity and strong stress resistance, the scarcity of male‑sterile materials means that production still relies on manual emasculation and pollination. This labor‑intensive process has bec...

Comprehensive Evaluation and Transcriptome Analysis of Drought Resistance in Castor Bean (Ricinus communis L.)

Castor bean (Ricinus communis L.) is an important industrial oil crop worldwide. In China, western Jilin Province represents a major production region for this species, where spring drought severely constrains its yield and quality.This study aimed to comprehensively evaluate drought resistance in castor bean and explo...

Transforming Plant Viruses into Vectors for Next-Generation Agriculture-A Review

CRISPR-Enabled functional genomics in hPSCs-derived neural models for autism spectrum disorder

This review synthesizes the current state of CRISPR-based functional genomics in human stem cell neural models as a coherent experimental framework for converting ASD genetic associations into mechanistic understanding and therapeutic opportunity.

Functional disruption of vgsc reveals haplosufficiency with implications for insecticide resistance and genetic control in Anopheles gambiae

The insecticide resistance phenotype of vgscKO is investigated and it is demonstrated that knocking out one copy of vgsc produces reduced susceptibility to deltamethrin and DDT at sub-discriminating doses, with implications for vgsc-mediated mechanisms of insecticide resistance.

CRISPR-enhanced assessment of variants of unknown significance nominates oncology therapeutic targets and drug repositioning opportunities

CRISPR-VUS combines dependency-based rare-variant discovery with evidence-guided prioritisation to nominate candidate drivers, therapeutic targets and drug-repositioning hypotheses to nominate candidate drivers, therapeutic targets and drug-repositioning hypotheses.