gene editing

Climate-resilient rice crop improvement using CRISPR–Cas technology

A RUBY-Based Visual Hairy Root Transformation and CRISPR-PTG Genome-Editing System in Eucommia ulmoides

A RUBY-based visual hairy root genetic transformation system was established, which provided preliminary evidence that CRISPR-PTG-mediated targeted mutagenesis can occur in E. ulmoides hairy roots, and a reference for genetic transformation studies of other woody plants.

Next-generation biotechnology for improving abiotic stress tolerance in rice

CRISPR/Cas9-Mediated Editing of Terpene Synthase Gene Reduces Volatile Emission and Attraction of Hypothenemus hampei to Coffea arabica Fruits

Results suggest that targeted editing of limonene synthase can modulate volatile emissions and alter host-seeking behavior in H. hampei, providing a proof-of-concept for engineering crop plants with reduced pest attraction and offering a complementary, sustainable strategy for pest management in coffee production syste...

Timed Self-Excision of PlWUS-IPT Resolves the Transformation Efficiency-Phenotype Trade- off in Tobacco

Tissue engineering: methods for scaffolds to extracellular matrix and scaffold to cell interaction

Engineering circular RNA expression systems to minimize contaminating linear RNA byproducts

Circular RNAs (circRNAs) are generated by backsplicing of eukaryotic protein-coding transcripts and can regulate microRNAs and RNA binding proteins, or serve as translation templates. Their covalently closed structure confers resistance to exonuclease-mediated degradation, extending their half-life and supporting their...

Evaluation of Alcalase pretreatment for Chlamydomonas reinhardtii CRISPR knock-in

We wanted to use CRISPR-Cas9-mediated homology-directed repair (HDR) in C. reinhardtii to insert genomic landing pads as part of a larger project. In cell-walled algae, delivering Cas9 ribonucleoproteins (RNPs) and donor DNA requires first digesting that wall. The standard approach uses autolysin, a gamete-derived prot...

Beyond Precision: A Multidimensional Framework for Selecting Genetic Medicine Platforms

A multidimensional framework is proposed in which gene therapy platforms are evaluated according to three intrinsic properties—genetic precision, temporal control, and dosage tunability—while delivery, clinical maturity, and disease context act as major translational constraints.

Lipoprotein(a) as an independent cardiovascular risk factor

Emerging evidence links Lp(a) to atrial fi brillation, broadening its clinical relevance and integrating Lp(a) testing into risk assessment and preparing for targeted therapies are critical steps in modern cardiovas-cular prevention.

An inducer-independent, single-plasmid CRISPR-Cas9 system for genome editing in Bacillus species

A one-step CRISPR-based transformation protocol developed in this study enables fast genome editing workflows with minimal hands-on time and demonstrates the versatility of the modified system for this industrially important genus.

The Cucurbita ficifolia Mitochondrial Genome: Repeat-Rich Architecture, Plastid Homology, and Synteny Turnover Across Cucurbitaceae

Read evidence supports the submitted terminal-to-start adjacency but does not establish a unique or predominant circular molecule in vivo; likewise, the largest repeat showed no robust reciprocal-junction signal.