Avian pathogenic Escherichia coli (APEC) causes substantial economic losses in the poultry industry, and vaccines capable of providing protection across diverse APEC strains remain needed. In this study, a FimH-CsgA-OmpA multiepitope fusion antigen (MEFA) was constructed and evaluated for immunogenicity, in vitro anti-...
Jia-Yu Cui, Ming-Qing Shao, Long-Long Wang et al.· Life· 0 citations
This work engineered a full-structural-protein virus-like particle co-assembled with PEDV S, M, N, and E proteins into uniform nanoscale particles, further formulated with a dual-adjuvant system of AS03 and CpG ODN and conferred robust protection against PEDV challenge in piglets.
Fei-Yang Jiang, Lian-Mei Bai, Yan-Yuan Zhang et al.· ACS Applied Materials and In...· 0 citations
Abstract: Classical AI answers from inside a representation fixed before the question arrives. What that representation can express, the system can answer; what lies outside it, no amount of parameters, data, search, or compute reaches. AlphaFold predicts through a learned representation; AlphaEvolve searches a space o...
Denise Holt, Denis Ovseyenko· Zenodo (CERN European Organi...· 0 citations
In this investigation, the antioxidant capacity of stirred-type hypoallergenic and lactose-free yogurt was improved using cylindra-type beetroot peel extract. Milk proteins were hydrolyzed with papain at different concentrations (0.008 g·L−1 and 0.012 g·L−1) and, subsequently, milk with papain-hydrolyzed proteins were...
Kenbon Beyene Abdisa, Moh Moh Zin, Adrienn Vargáné Tóth et al.· Applied Sciences· 0 citations
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Abstract Alternative splicing (AS) is dysregulated in pediatric CNS tumors, including high-grade gliomas (HGGs) and atypical teratoid/rhabdoid tumors (ATRTs) which have poor survival outcomes and urgently need new therapeutic strategies. AS is increasingly being explored as a source of tumor-specific antigens for immun...
Ryan Corbett, Meghali Goswami, Patricia Sullivan et al.· Cancer Research· 0 citations
We present a theoretical and numerical study of protein folding kinetics and conformational transitions in complex free-energy landscapes using generalized Langevin equations with memory kernels and Fokker-Planck formalisms. Moving beyond standard deterministic approaches, we investigate non-equilibrium folding dynamic...
Henrietta Volkova· Zenodo (CERN European Organi...· 0 citations
Due to rising drug resistance, novel therapeutics are needed to combat the causative agent of malaria, Plasmodium spp.. Aspartic protease inhibition has emerged as a promising frontier for anti-malarial development as P. falciparum expresses five essential aspartic proteases in its symptomatic erythrocytic stages. Thes...
Katherine Floyd· Washington University in St....· 0 citations
A short procedure for assembling Cas9 RNPs with in vitro-transcribed guide RNAs is provided, making the protocol directly applicable to CRISPR RNP delivery in mammalian cells.
M. Skrbinek, Jure Bohinc· Methods in molecular biology· 0 citations
Dogs with PCC exhibit a distinct circulating protein signature vs healthy controls, providing clues to PCC pathogenesis and nominating proteins as diagnostic biomarker candidates and potential therapeutic targets.
Marit F. van den Berg, A. Rodríguez-Rojas, P. Doulidis et al.· Journal of Veterinary Intern...· 0 citations
MT-ProtBERT consistently outperforms PARROT, an RNN-based IDP-specific model, across all tasks, and demonstrates that combining self-supervised and biochemistry-informed tasks, and multi-scale learning enables robust modeling of unstructured proteins under data scarcity.
Jian Sun, Kingshuk Ghosh, Lilianna Houston et al.· 0 citations
Nisin is a widely used antimicrobial peptide with strong inhibitory activity against Gram-positive bacteria and growing interest for broader biomedical applications. However, its practical use is limited by susceptibility to environmental conditions, reduced stability, and rapid inactivation. Encapsulation has emerged...
Mesophilic enzymes are often less active at low and moderate temperatures than their psychrophilic homologues. The earthworm Eisenia fetida has cold-adapted enzymes, but the end-β-1,4-mannanase shows weaker activity at low temperatures compared with other E. fetida enzymes. Cold-adapted enzymes need flexible structur...
Yu Hirano, Yuki Naka, Yuya Hanazono et al.· Journal of Agricultural and...· 0 citations
A new machine-learning framework aims to improve the success rate of computational protein design while moving away from results that reproduce sequences found in nature.
