protein folding

The Mystery of the 10⁵ Temperature Gap Inside Cells: The Limits and Challenges of Nanothermometry

Researchers at Osaka University in Japan and the University of Queensland in Australia have published a review analysing the enormous gap between theory and measured values that confronts luminescence nanothermometry, the technique used to measure temperature inside cells. The authors argue that the concept of temperat...

Integrated Time-Series Biochemical, Transcriptomic and Metabolomic Analyses Reveal Key Pathways and Concentration-Dependent Transitions in Chironomid Larvae (Propsilocerus akamusi) Under Chlorantraniliprole Stress

Findings reveal the adaptive strategies of chironomids to cope with pesticide stress from the environment, and highlight the urgent need to protect these animals and their freshwater habitats from agricultural pollution.

Predicting Spatial Variability of PR Protein Concentration in White Grape Juice Using UAV Multispectral Data and Machine Learning

Background: Pathogenesis-related (PR) proteins, primarily thaumatin-like proteins (TLPs) and chitinases, are the principal cause of protein haze formation in white wines, increasing bentonite requirements and affecting winemaking efficiency. However, evaluating their spatial variability before harvest remains challengi...

CAF-1 in Replication and Repair: A Critical Genetic Mediator of Synthesis-Coupled Nucleosome Assembly

Current advances in understanding the molecular mechanisms by which human and yeast CAF-1 complexes are recruited to sites of DNA synthesis and how CAF-1 function is coordinated with other histone chaperones during replication and repair are summarized.

Improvement of functional and antioxidant properties of Sacha inchi protein through glycosylation with chitosan oligosaccharide

Comprehensive analysis of non-synonymous single-nucleotide polymorphism of human TSC1 and TSC2 genes: An in silico approach

Abstract Tuberous sclerosis complex (TSC) is an autosomal dominant disorder caused by mutations in the TSC1 and TSC2 genes and is characterized by benign hamartoma formation in multiple organs. The TSC1–TSC2 complex regulates mTORC1 signaling in response to cellular growth conditions. This study aims to predict the str...

Discovery and Characterization of a Novel Ligand-Binding Site in UFC1 Using Sulfa- and Sulfonate-Based Compounds

UFMylation is an important biological process where proteins are post-translationally modified via the covalent attachment of the ubiquitin-like modifier (UBL), ubiquitin-fold modifier-1 (UFM1). UFMylation is analogous to ubiquitination, occurring via the transfer of UFM1 across E1-, E2-, and E3-like enzymes, represe...

Oncogene activation mechanism determines the limits of targeted protein degradation.

This work developed orthogonal methods to increase production or enhance stability of β-catenin, an important oncoprotein and target for degrader therapeutics, and used the dTAG system to evaluate the consequences for PROTAC activity.

DNM2–Charcot–Marie–Tooth neuropathy stems from disrupted Schwann cell function and shows limited therapeutic reversibility

These findings establish the Dnm2K562E/SC− mouse as a robust preclinical model, recapitulating key features of DNM2–Charcot–Marie–Tooth, and provide crucial insight into the biological and temporal constraints that must guide future therapeutic strategies for DNM2–Charcot–Marie–Tooth.

Image-based and biochemical multimodal phenotyping for explainable classification of chia (Salvia hispanica L.) genotypes.

The findings indicate that multimodal phenotyping, coupled with explainable machine learning, offers a practical and biologically interpretable decision-support approach for chia genotype classification.

Soy Protein Isolate-β-Cyclodextrin Microencapsulation Boosts Stability and Intestinal Delivery of α-Linolenic Acid-Rich Structured Lipids: Oxidation, Digestion, and Molecular Docking Evaluation.

In vitro simulated digestion demonstrated its characteristic intestinal sustained-release behavior, which provides preliminary in vitro evidence that may favor enhanced intestinal absorption of α-linolenic acid (ALA); however, further cellular or animal trials are required to quantitatively validate its actual in vivo...

β-strand addition within tip initiation complexes licenses assembly of diverse type IV filaments

How PilC/PilY1 can be retained on the fiber tip under enormous tensile loads generated during mechanical shear and T4P retraction is explained and how diverse T4P systems employ β-strand addition to license fiber assembly is demonstrated.