protein folding

Dietary astaxanthin alleviates microcystin-LR-induced hepatopancreatic digestive and absorptive dysfunction in red claw crayfish (Cherax quadricarinatus)

Astaxanthin is a functional feed additive with antioxidant and antistress properties in crustaceans. This study investigated the protective efficacy and underlying mechanisms of dietary astaxanthin against hepatopancreatic digestive and absorptive dysfunction caused by microcystin-LR (MC-LR) in red claw crayfish ( Cher...

Machine learning-assisted directed evolution yields dramatic improvement on novel AAV engineering task

Directed evolution enables the discovery of protein mutants with improved fitness through iterative rounds of selection and has been widely applied to adeno-associated virus (AAV) capsid engineering. Machine learning (ML) can augment this process by prioritising mutants for experimental validation, but whether its bene...

Triple-Omics Analysis Unveiled the Neuroprotective Mechanisms of Akkermansia muciniphila in an Environmental Exposure-Linked Mouse Model of Alzheimer’s Disease

Cadmium (Cd) is an emerging environmental risk factor for cognitive decline and Alzheimer’s disease, and few interventions limit its effects on the brain. Akkermansia muciniphila, a human gut commensal, is neuroprotective through mechanisms that remain unclear. Building on our finding that A. muciniphila prevented Cd-i...

Rapid metabolic control after primary islet transplantation: relevance for stem cell-derived islet product development

oxygenation before imposing a high secretory demand on the engrafted β-cells (2). Since primary islets isolated from deceased donors-derived islets (also referred to as primary islets) are developmentally and functionally mature, they continue to sense glucose and secrete insulin immediately post-transplant, despite th...

Atomic Level Characterization of PolyGlycine Assembly

Polyglycine peptides can adopt polyproline II (PPII) helical conformations that assemble into honeycomb-shaped oligomers. These polyglycine II (PGII) helical bundles, which are also found in several natural proteins, lack apolar groups and cannot be stabilized by hydrophobic interactions. Thus, their folding must diffe...

CD248 expression in human adipose tissue is upregulated in obesity and following glucocorticoid exposure: a mediator of insulin resistance and adipose dysfunction?

CD248 is a transmembrane glycoprotein implicated in fibrosis, hypoxia, and insulin resistance, but its role in human adipose tissue remains incompletely understood. Because glucocorticoids regulate these processes, we investigated whether CD248 is glucocorticoid responsive and characterized its expression across adipos...

The processing class of the second residue is conserved across three domains of life, and retention of the initiator methionine is coupled to packing into the protein fold

Molecular remodelling of Cryptococcus neoformans during adaptation to host temperature

The molecular and metabolic responses of the C. neoformans H99 strain grown at 30 and 37 °C are investigated using an integrated transcriptomic, proteomic and functional approach, revealing a more selective response.

Polar vs Non-polar Excipients in BSA Aggregation.

PURPOSE In recent years, there has been a rapid expansion in the development of new protein therapeutics in the pharmaceutical industry. Problematically, proteins are conformationally and colloidally marginally stable, and thus it is necessary to formulate them using buffering and stabilizing agents. METHODS In this...

Divergent interfaces and convergent outcome in the structural basis of HIV-2 Vif antagonism of APOBEC3H

Primate lentiviruses counteract antiviral APOBEC3 (A3) cytidine deaminases through virion infectivity factor (Vif), which recruits A3 proteins to a Cullin–RING E3 ubiquitin ligase for proteasomal degradation. While HIV-1 Vif is well characterized, HIV-2 Vif remains poorly defined due to divergent sequences. Here we r...

BCL2A1 marks an early, less-exhausted CD8+ tumor-infiltrating lymphocyte state associated with PD-1 blockade response in NSCLC

The tumor microenvironment exerts intense survival pressure on CD8 + tumor-infiltrating lymphocytes (TILs), yet the cellular basis for an earlier, less-exhausted state associated with response to immune checkpoint blockade (ICB) in non-small-cell lung cancer (NSCLC) remains unclear. We previously identified BC...

Observed Clinical and Operational Factors Are Associated with Biomarker Test Utilization in Respiratory Virus RT-PCR Testing: A Real-World Analysis of Inflammatory, Cardiac, and Thrombotic Biomarkers

Background/Objectives: Routinely collected biomarker data are generated through selective testing. We examined associations of observed clinical and operational factors with biomarker availability and explored the incremental discrimination provided by biomarker concentrations for respiratory viral RT-PCR positivity. M...