Nanopore sensors have demonstrated single-molecule resolution for nucleic acids and small molecules, yet the discrimination and detection of folded proteins in complex biological matrices remains a challenge. Insulin is a demanding test case: it circulates in blood at picomolar concentrations, differs from clinically...
The thermophilic filamentous fungus Humicola insolens is a rich reservoir of enzymes with broad application potential, owing to their thermostability and neutral pH optima. H. insolens is also distinguished in secreting abundant catalase. Despite this characteristic, its potential as a host for heterologous gene expres...
Shuyan Yang, Chao Fan, Xianhua Sun et al.· Microbial Cell Factories· 0 citations
Green coconut mesocarp is a substantial agro‐industrial waste, and its valorization can be achieved by extracting the industrially valuable enzyme peroxidase (POD). Proximate analysis of the mesocarp revealed a protein content of approximately 2.5% (wet basis). The extraction was conducted using 0.5 M sodium phosphat...
P. M. Vikram, Shraddha Yewate, Vishal Kalambe et al.· Biofuels, Bioproducts and Bi...· 0 citations
Crystal structures provided a framework for further investigation of NTD–RNA recognition, the possible role of the CTD positively charged groove in nucleic acid interactions and N-protein assembly and suggest that RNA binding may be accommodated by local side-chain rearrangements within a pre-existing positively charge...
Xiao-Jing Song, G. Chalhoub, Peter J. McCormick et al.· Acta Crystallographica Secti...· 0 citations
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These findings provide a comparative molecular-pathology framework for interpreting NOTCH3 variation beyond cysteine number alone and support a context-dependent model in which residue position, disulfide architecture, surrounding sequence, domain organization, aggregation propensity, and proteolytic regulation jointly...
K. S. Siddiqui, H. Ertan, Yi Ren et al.· bioRxiv· 0 citations
The sensitivity of ovarian nervous system cells to MEHP offers new mechanistic insights into reported links between phthalates, altered ovarian sensitivity index, and polyendocrine metabolic ovarian syndrome, and the need for a holistic assessment of ovarian toxicity that considers all ovarian cell types, not only foll...
E. M. Panagiotou, Loren Méar, I. Rooda et al.· EBioMedicine· 0 citations
Tuberculosis remains among the top ten deadliest infectious diseases worldwide, primarily due to delayed diagnosis, co-infection with HIV, and multidrug resistance. Targeting early secreted mycobacterial antigens, including MPT64, ESAT6, and CFP10, may be a viable strategy for detecting the onset of tuberculosis. Altho...
Lucas Sekele, Adeniyi T. Adewumi, Amanda Skepu et al.· Protein Expression and Purif...· 0 citations
It is demonstrated with confocal microscopy that reflectins A1, A2, B and C individually undergo LLPS to form protein dense liquid condensates, while physiological mixtures of these proteins exhibit coordinated phase transitions to form multiphase condensates whose internal spatial organization is tuned by the proporti...
Reid Gordon, R. Levenson, Brandon Malady et al.· Biophysical Journal· 0 citations
In adults aged 60 years or older, RSVpreF vaccination reduced hospitalisations for RSV-related respiratory tract disease with no statistical evidence of effect modification by immunosuppression status; however, the study was not powered to exclude subgroup differences.
A. Reimer Jensen, M. H. Højbjerg Lassen, N. Johansen et al.· The Lancet Healthy Longevity· 0 citations
The results extend de novo antibody design from binary antigen-antibody complexes to programmable multi-component complexes, providing a foundation for designing single molecules that combine recognition of distinct targets and their associated biological functions.
BACKGROUND
Infants with non-specific symptoms are sometimes labelled as cow's milk allergic and managed with specialized formula. These products differ from standard infant formula in protein and carbohydrate source, typically containing lower quality protein and free/added sugars such as glucose polymers or sucrose. W...
N. Punjabi, Karen Li, Mark Cunningham et al.· Clinical and Experimental Al...· 0 citations
AlphaFold 3 predicts protein structures with remarkable accuracy, yet how structural information emerges within the model remains poorly understood. Here, through causal interventions on internal representations and direct probing of every Pairformer block, we trace the formation of global protein geometry and identify...
A new machine-learning framework aims to improve the success rate of computational protein design while moving away from results that reproduce sequences found in nature.
