Open access

Integrated single-cell and bulk transcriptomic analysis reveals an epithelial cell-derived prognostic and immunotherapeutic signature with ADAM12 as a key functional regulator in non-small cell lung cancer

Hai-Lin Liu Jun-Jie Yu Yan-Tao Jiang Xiao-Fei Ma Wei Luo Shu-Yue Guo Lu-Yao Tong Yi Lu Zhen-Fa Zhang Chen-Guang Li Jian-Wei Qi Ting-Ting Qin

Sep 2026 · Translational Oncology · Vol 73 · 0 citations · 48 references

Medicine

Abstract

Highlights • Single-cell transcriptomics reveals epithelial heterogeneity shaping NSCLC immunity.• A 101-algorithm ML framework constructs a Prognostic and Immunotherapeutic Signature.• PIS stratifies NSCLC patients by prognosis and immune checkpoint blockade response.• ADAM12 inhibition enhances anti-PD-1 efficacy in preclinical mouse models.

DOI PubMed Semantic Scholar

Read PDF

Similar papers

Open access Aug 2026

Integration of single-cell transcriptomics and genomic mutation analysis identifies an immunotherapy-resistant tumor subcluster and validates ARNTL2 as a malignant driver in lung adenocarcinoma

Single-cell sequencing reveals resistance-linked malignant subcluster in LUAD, confirming oncogenic role and CoxBoost + survivalSVM model achieves optimal C-index of 0.686 across seven cohorts.

Z. Qiu, Hui Zhao, Qiong Lyu et al. · Translational Oncology · 0 citations

Open access Aug 2026

Integrative single-cell sequencing and machine learning analyses identify chronic inflammation and cancer-associated fibroblast subsets as drivers of adverse outcomes in ovarian cancer

Single-cell analysis identifies M8 fibroblast subcluster as dominant post-NACT population linked to ovarian cancer chemo-resistance and NHP2 knockdown suppresses proliferation, migration and invasion of ovarian cancer cells in vitro.

Mingqin Kuang, Chang-Mei Shen, Chun-Ping Yang et al. · Translational Oncology · 0 citations

Open access Aug 2026

Integrated single-cell and bulk transcriptomic analysis identifies a novel senescent fibroblast subtype associated with poor prognosis in acral melanoma

Highlights • A high-resolution single-cell atlas of acral melanoma was constructed, revealing 10 major cell types and 16 melanoma subpopulations.• A novel senescent fibroblast subtype (sCAF_CDKN2A) was identified as a key driver of poor prognosis in acral melanoma.• SEMA6A, KIT, COL3A1, and VCAN were identified and val...

Ming Jing, Zhenyu Xu, Zhenqiang Ruan et al. · Translational Oncology · 0 citations

Open access Aug 2026

Single-cell and bulk transcriptomic profiling of M2-like tumor-associated macrophages reveals a prognostic signature and an inflammation–metabolism crosstalk program in multiple myeloma

Highlights • Seven MM-specific M2-TAM subsets (MM-M2) are absent in normal bone marrow.• MM-M2 subclusters exhibit distinct transcription factor programs via SCENIC.• Enhanced ligand–receptor signaling links MM-M2 to plasma and stromal cells.• An 11-gene RSF-derived MRGs signature stratifies MM patients by survival.• M...

Hui Zhao, Jing Kang, H. Ouyang et al. · Translational Oncology · 0 citations

Open access Aug 2026

Integrative single-cell and machine-learning analysis identifies LGALS1 as a macrophage-associated diagnostic and prognostic biomarker in hepatocellular carcinoma

Highlights • Single-cell and machine learning defined macrophage-driven LIHC subtypes.• LGALS1 was identified as the top SHAP-ranked therapeutic evasion driver.• LGALS1 was enriched in macrophage and fibroblast lineages in LIHC.• LGALS1 silencing impaired HCC growth, migration, invasion, and clonogenicity.

Yi Wan, Qiang Huo, Yi-Jie Yang et al. · Translational Oncology · 0 citations

Open access Aug 2026

TMEM176B orchestrates intratumoral hierarchy and immunosuppression in hepatocellular carcinoma: Insights from single-cell transcriptomics

High-resolution mapping of 52,384 cells uncovers hierarchical intra-tumor heterogeneity in HCC and TMEM176B is identified as the link between tumor malignancy hierarchy and immune remodeling.

Hong Chen, Jian-Feng Gong, De-Ying He et al. · Translational Oncology · 0 citations