A genome-wide CRISPR screen in human prostate cancer cells reveals drivers of macrophage-mediated cell killing and positions AR as a tumor-intrinsic immunomodulator

In vivo genome-wide CRISPR screens identify FOXR1 as a suppressor of CD8+ T cell antitumor immunity.

T cell dysfunction critically limits the efficacy of T cell-based immunotherapies in solid tumors, yet the intrinsic regulators of T cell dysfunction remain incompletely understood. Through an in vivo genome-wide CRISPR screen in tumor-infiltrating CD8+ T cells, we identified Forkhead Box R1 (FOXR1) as a potent transcr...

CRISPRa-identified transcription factors reprogram glioblastoma into dendritic cell-like cells to elicit systemic antitumor immunity

A candidate-based CRISPR activation (CRISPRa) functional screen is developed to identify regulators of conventional dendritic cell (cDC)-fate specification and concludes that the transcription factors Zfp366/Znf366, Pu.1, Irf8, and Batf3 (ZPIB) are sufficient to convert GBM cells into cDC-like cells.

Tumor microenvironment-mediated resistance to immune checkpoint inhibitors in non-small cell lung cancer: Mechanisms, combination strategies and clinical perspectives (Review)

Non-small cell lung cancer (NSCLC) is one of the leading causes of cancer-associated mortality worldwide. Immune checkpoint inhibitors targeting programmed cell death protein 1, programmed death-ligand 1 (PD-L1) and cytotoxic T lymphocyte-associated protein 4 have transformed the treatment landscape of NSCLC. However,...

CRISPR Screen and Single-Cell Multi-Omics Identify a Novel Immune Checkpoint on Tumor-Infiltrating Regulatory T Cells

Tumor infiltration regulatory T cells (Tregs) mediate immune escape by highly expressing multiple inhibitory receptors, which is one of the key factors in resistance to existing immune checkpoint inhibitors. However, systematic screening of Treg-specific immune checkpoints within tumors remains lacking. This study aims...

Genome-wide CRISPR screens identify an RXR–MYLIP–LDLR axis regulating BH3 mimetic sensitivity in peripheral T-cell lymphomas

Integrated spatial and single-cell transcriptomics uncover IFI6⁺ regulatory T-cells and SPP1⁺ macrophages immunosuppressive niches in colorectal cancer.

This study uncovered previously unrecognized immunosuppressive cell populations and key immune regulators that advance understanding of CRC immune evasion and highlight potential targets for personalized immunotherapy.