Abstract Cas9 is a metal-dependent nuclease used for genome editing across diverse cells and organisms exhibiting distinct ionic environments, yet how metal ions regulate its catalytic function, and consequently its editing efficiency remains unclear. Here, molecular simulations, Markov state models, and mixed quantum–...
Mohd Ahsan, Alexa L Knight, Aakash Saha et al.· Nature Communications· 2 citations
Progress in mouse genetics, CRISPR/Cas-based genome editing, conditional alleles, and next-generation humanized platforms has expanded the toolkit for functional interrogation of host pathways that shape sepsis outcomes, and can link genotype-to-phenotype mechanisms with biomarker-informed therapeutic testing.
B. Abbas, Arshad Abbas, Iqbal Nawaz Khan et al.· Clinical Immunology· 0 citations
Two-pore domain potassium (K2P) channels are important regulators of the resting membrane potential in various cell types. They are conserved across different phyla and are involved in a wide range of physiological processes and biological functions (Chapter 1). The K2P gene family is greatly diversified and expanded i...
CRISPR-Cas12a holds great promise for molecular diagnostics and gene editing due to its sequence-specific DNA recognition and robust trans-cleavage activity, yet precise tuning of its nuclease activity remains challenging. Herein, we report a simple temperature-regulation strategy using cost-effective ssDNA blockers, t...
Tartary buckwheat (F. tataricum) is a climate-resilient pseudocereal, yet its global adoption is constrained by its bitter seeds. Here, we employed the CRISPR/Cas9 system to disrupt the rutin-degrading enzyme 2 gene (FtRDE2), which catalyses the hydrolysis of rutin to quercetin, a major contributor to bitterness. Two i...
Artur Piński, Magdalena Zaranek, Joanna Lusinska et al.· Journal of Experimental Bota...· 0 citations
A correction refers to a change to their article that the author wishes to publish after publication. The publication of this article is subject to Frontiers' editorial approval.• Please read through all the templates before choosing • Pick the most relevant text template(s) from the following page and delete all other...
Mao Liu, Haochen Sun, Qun Yao et al.· Frontiers in Immunology· 0 citations
In November 2025, the Government of India launched BIRSA 101 (Bhagwan Birsa Munda), the country's first indigenous CRISPR-based gene-editing therapy for sickle cell disease (SCD), with the aim of a one-time curative approach.1 It was developed by CSIR-Institute of Genomics and Integrative Biology (CSIR-IGIB), with tech...
Sarbasree Bhattacharjee, Jarina Begum· The Lancet Regional Health -...· 0 citations
Sorghum bicolor (L.) Moench is a valuable cereal crop for tackling micronutrient malnutrition globally through biofortification of essential nutrients, iron (Fe) and zinc (Zn). Sorghum seeds exhibit significant variability in Fe and Zn content, yet only two biofortified varieties have been released so far. In this st...
G. Yadav, Payal Jangra, Khushi Jangra et al.· ACS Agricultural Science &am...· 0 citations
Los síndromes displásicos complejos neuro-óseo-mucocutáneos son un grupo de enfermedades genéticas raras causadas por defectos en genes que regulan el desarrollo neuroectodérmico y mesodérmico, afectando con frecuencia el sistema nervioso central, el hueso craneofacial, la piel y la mucosa oral. El tratamiento actual e...
Jhoselyn Vanessa Yucailla Rojas, Federico Angulo Micolta, Robinson Giordano Pilamunga Sisa et al.· Salud Medicina e Innovación...· 0 citations
Schwann cells are vital to development and maintenance of the peripheral nervous system, and their dysfunction has been implicated in a range of neurological and neoplastic disorders, including
NF2
-related schwannomatosis (
NF2
-SWN). We have developed a human induced pluripotent stem cells (hiPSCs) model for...
Olivia Lazaro, Si-Hong Li, Jacob S. Smiley et al.· Scientific Reports· 0 citations
Lactococcus cremoris
is an emerging microbial chassis for food-grade and therapeutic protein production. However, existing promoter collections available for
L. cremoris
provide only a limited dynamic range, thereby constraining applications that require high transcriptional output without chemical inducers. H...
Javier Nicolás Garay-Novillo, J. Ruiz-Masó, Tatiana Valeria Guendulain et al.· Applied Microbiology and Bio...· 0 citations
Phenylketonuria (PKU) is an inherited metabolic disorder caused by mutations in the
Pah
gene, resulting in impaired phenylalanine hydroxylase activity and elevated blood phenylalanine levels. Although dietary management and enzyme replacement can reduce disease burden, they require lifelong treatment and do not c...
Zero-knowledge proofs can verify computing results without rerunning workloads or exposing sensitive data. Magnetar helps bring them into the mainstream with support for standard programming languages, major speed gains, and quantum-resistant security. The post Proof-based general-purpose computing with Magnetar appeared first on Microsoft Research.
Exploring how generative AI could make machine vision more accessible to businesses. The post GenEye in a Box: Making Machine Vision Something You Can Just Ask For appeared first on GPT-Lab.
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