gene editing

Impact of the atmospheric air pollution level and xenobiotic biotransformation systems on the incidence of breast cancer

An increase in formaldehyde concentration in the air is associated with a gain in breast cancer incidence, especially at the early stages, and it is advisable to strengthen formaldehyde monitoring, implement molecular epidemiological screening of these polymorphisms and active detection of breast cancer in genetically determined risk groups.

Light- and X-ray-Activated Liposomes for Controlled Gene Editing and Drug Delivery.

This protocol presents methods for fabricating and characterising two distinct verteporfin (VP)-integrated lipid nanoparticle formulations and demonstrates knockout of up to approximately 326 slow-muscle fibres per zebrafish embryo via light activation and significant tumour growth suppression via X-ray-triggered doxorubicin release.

A Toolbox for Binary and Rheostat-like Modulation of TOX Expression via Genome and Epigenome Editing in Primary Human T cells

These studies show that a complete TOX knockout or silencing has no effect on exhaustion marker expression levels or the transcriptome in repeat-anti-CD3/anti-CD28-stimulated primary human T cells in vitro.

Triple potentiator combinations increase the functional correction of several CFTR gating mutants

The findings suggest that triple potentiation can significantly enhance functional restoration of poorly responsive gating mutants, thereby uncovering novel avenues for therapeutic development.

Transcriptomics of independent CRISPR-edited cell lines reveal ciliary-specific ARL13B dependent changes

It is demonstrated that ciliary ARL13B is required to maintain normal ciliary composition and gene expression programs and underscores the value of multi-clone, rescue-based experimental designs for robust transcriptomic analyses.

Liver Organoids: From Disease Modelling to Regenerative Medicine

This review systematically summarises current fabrication strategies, disease‐modelling utilities and regenerative potentials of liver organoids, alongside the major challenges and future directions.

CRISPR-Cas9 Precision Breeding for Climate-resilient Vegetable Crops: A Critical Appraisal of Evidence, Inference and Deployment

Confidence in current claims of climate resilience remains low for most targets, and progress will depend on multi-environment field testing, yield-based endpoints, and extension of editing capability beyond the few genotypes that presently regenerate reliably.

CRISPR/Cas9-Mediated Site-Directed Mutagenesis of Genes in Klebsiella pneumoniae.

This protocol minimizes off-target editing, shortens experimental timelines, reduces screening workload, and provides a reliable platform for investigating resistance mechanisms, validating candidate genes, and supporting functional genomics studies in clinically relevant bacterial pathogens.

Erratum: Resolution of hepatic fibrosis after ZFN-mediated gene editing in the PiZ mouse model of human α1-antitrypsin deficiency.

Overcoming Therapy Resistance in Ovarian Cancer: From Molecular Mechanisms to Emerging Therapeutic Strategies

This review summarizes emerging therapeutic strategies for EOC, their mechanisms of action, and their potential to overcome treatment resistance, and covers molecularly targeted therapies, immunotherapies, metabolic and epigenetic approaches, cellular and gene therapies, targeted drug-delivery systems, and locoregional and physical modalities.

Oncorhynchus mykiss as a Salmonid Functional Genomics Model: A Structured Narrative Review of Genomic Resources, Mucosal Immunity, Disease Resistance, Environmental Stress, and Causal Validation

Integration of pangenomics, regulatory annotation, single-cell and spatial analyses, and paralogue-aware functional perturbation will strengthen causal inference, clarify genotype–phenotype relationships, and support disease-resistant breeding, healthy aquaculture, and environmental risk assessment.

Stem cell–based therapies in pediatric disorders: translational advances, unresolved challenges, and future horizons

This paper will provide the current information on stem cells used in the treatment of children and the many different types of stem cells, including: hematopoietic stem cells (and their derivatives), mesenchymal stem cells (and their derivatives), induced pluripotent stem cells, embryonic stem cells, tissue-specific progenitor cells, extracellular vesicles, and bioengineered products.