gene editing

Integrative Techniques for Hybrid Breeding in Eucalyptus: Advances, Innovations, Challenges, and Future Prospects

The aim of this review is to present integrative techniques for hybrid breeding in Eucalyptus based on conventional methods such as phenotypic selection and controlled hybridization, and modern genomic-assisted approaches to guide product development.

Universal donor plasmids for biallelic degron-tagging in mammalian cells and organoids

A series of universal donor vectors are generated to enable biallelic integration of different degron tags using different selection strategies (FACS, drug selection) and can be fused in-frame to endogenous protein C-termini via homology-independent repair following Cas9-induced double strand breaks.

Interrogation of noncoding schizophrenia risk variants using CRISPR-based functional genomics

Findings show distinct noncoding mechanisms that map within GWAS signals, and provide a path forward for interrogating noncoding regulatory elements and variants in disease loci.

Parthenolide boosts megakaryocyte maturation and restores platelet responses in Wiskott–Aldrich syndrome

Wiskott-Aldrich syndrome (WAS) is an inborn error of immunity with a broad disease spectrum, classified into class I (late onset) or II (early onset) variants. Thrombocytopenia and small platelets are the most consistent findings among patients, difficult to treat and related to development of autoimmunity. To identify...

Infectious transgenesis in arthropods

In the last decade, there has been an explosion in the development of in vivo germline genetic editing technologies in arthropods, first pioneered by the Receptor-Mediated Ovary Transduction of Cargo (ReMOT Control) method. While ReMOT Control and related technologies (DIPA-CRISPR and SYNCAS) have shown great utility f...

Nanomedicine‐driven innovations in postoperative cancer immunotherapy: Remodeling tumor microenvironment and precision delivery strategies

Postoperative recurrence remains a critical challenge in cancer management, necessitating innovative strategies to reshape the tumor microenvironment (TME) and enhance immune surveillance. This review highlights nanomedicine‐driven breakthroughs in postoperative immunotherapy, emphasizing their potential to overcome...

A heuristic graph editing approach to correlation clustering with overlap

Correlation clustering seeks a partition of the vertex set of a given graph/network into groups of closely related, or just close enough, vertices so that elements of different groups are not close to each other. The problem has been previously modeled and studied as a graph editing problem, namely Cluster Editing , wh...

Gene editing using large insertions

Three experts in the field of programmable genome engineering systems using recombinases; transposases; transposases; genome writing systems; and retrotransposons promise different avenues for therapy, but each system has unique limitations.

Mitochondrial Quality Control in Inherited Mitochondrial Cardiomyopathy: Convergent Pathobiology and a Testable Therapeutic Framework

Inherited mitochondrial cardiomyopathies arise from pathogenic variants affecting oxidative phosphorylation, mitochondrial DNA maintenance, cardiolipin remodeling, protein import, cofactor metabolism, and mitochondrial dynamics or proteostasis. These disorders may be cardiac-predominant or part of multisystem disease....

Genetic principles enabling the creation of bacteria-based therapeutics.

This review examines the genetic design principles and liabilities of bacteria-based therapeutics, approaches used to produce clinical and near-clinical candidates, and the emergence of programmed bacteria delivering complete gene editing systems to therapeutically relevant cells.

Fusion of a non-specific DNA-binding domain enhances Cas12a trans-cleavage for robust nucleic-acid diagnostics

CRISPR–Cas12a underlies powerful genome-editing and nucleic-acid detection technologies, yet its performance is limited by inefficient target engagement and low catalytic turnover, particularly at low target abundance and elevated temperatures. Here, we report a modular protein-engineering strategy to enhance Cas12a tr...

Improved long-term proliferation and myogenic differentiation of bovine muscle stem cells through CDKN2A gene editing