protein folding

Common cis-eQTLs do not explain chromosome 21 expression deviations from the ploidy expectation in Down syndrome

Down syndrome (DS) results from trisomy 21 (T21), the triplication of human chromosome 21 (HSA21). On average, genes on HSA21 are overexpressed at the expected 1.5-fold increase. A small subset of HSA21 genes is expressed closer to euploid levels. Could local genetic regulatory variation explain deviations from the exp...

Session 7: Protein Folding and Degradation in Aging and Disease (joint session)

Heat Stress Responses During Grain Filling in Two Korean Wheat Cultivars as Affected by Developmental Stage and Exposure Duration

High temperature can impair grain development and storage compound accumulation during wheat grain filling, with responses depending on stress intensity, timing, duration, and cultivar. In this study, Saekeumkang and Hwanggeumal were selected from among major Korean wheat cultivars because they differ in grain quality-...

Flocculent Ti3AlC2-Based Two-Dimensional Material Flexible Microneedle Patch for Screening Alzheimer’s Disease Animal Biomarkers and In Situ Revealing Spatial Aggregation of Arachidonic Acid

Efficient diagnosis and pathological elucidation of Alzheimer’s disease (AD) are constrained by the lack of technologies for in situ capture of metabolites in living brain tissues. Metabolic disorders in the parietal lobe highly correlate with AD progression, yet existing methods cannot achieve in situ capture and spat...

Evaluation of Two Non-Invasive Extracorporeal Strategies for Enhancing Protein-Bound Uremic Toxin Removal During Hemodialysis

Current hemodialysis (HD) methods inefficiently remove protein-bound uremic toxins (PBUTs) due to their high binding affinity to plasma proteins, mainly albumin. This study aimed to investigate electromagnetic (EM) fields and temperature as potential extracorporeal, non-systemic methods to increase HD PBUT clearance. P...

Protein Language Models for Protein Classification: A Comparison with Sequence-Based Features

Protein language models learn contextual representations from large sequence collections, but theirpractical advantage over simple sequence-pattern features depends on the task and the evaluationsetting. This exploratory study compares frozen representations from the 8-million-parameterESM-2 model (facebook/esm2_t6_8M_...

DESIGNING AND SIMULATING SEQUENCE-ENCODED PHASE SEPARATION IN DISORDERED AND MULTIDOMAIN PROTEINS

Biomolecular condensates (BMCs) are membraneless organelles that can form through liq- uid–liquid phase separation. They help organize some of a cell’s biochemistry. It has been observed that improper formation of these condensates can be associated with neurodegenerative diseases and cancer. Much of the behavior assoc...

Utilization of grain milling waste for plant growth and nutrient availability in soil

This study optimized fermentation process of grain milling waste (wheat bran, wheat flour and mill dust) using Lactiplantibacillus plantarum (LP) or Bacillus subtilis (BS). The experiment included two parts: (I) in vitro optimization and evaluation of the fermentation of mill waste in two inoculum levels and th...

The Disordome Paradigm: How Intrinsic Disorder Encodes Emergent Biological Function

Classical structural biology equates function with a fixed three-dimensional fold, a view challenged by intrinsically disordered proteins (IDPs) and regions (IDRs), which populate broad conformational ensembles rather than a unique structure. This article introduces the “disordome” paradigm, extending Uversky’s “unfold...

Comprehensive O -Phosphoproteomic Landscape of Corynebacterium glutamicum

Corynebacterium glutamicum serves as a pivotal industrial platform organism, accounting for an annual global amino acid output of several million tons. Elucidating its adaptive mechanisms under diverse environmental perturbations during high-density fermentation is fundamental to advanced metabolic engineering and st...

Efficient deduction of encoded local response within a thermodynamically perturbed folded protein.

Externally imposed perturbations can subtly reshape protein structure and mechanics prior to the emergence of global unfolding signatures. Delineating locally sensitive zones, however, can pose immense challenges. Subjecting a small folded protein to isotropic pressure spanning 0.001-10 kbar, we develop a simplified an...

Protein Folding in the Cell