protein folding

Enzymatic routes to site-specific protein-nucleic acid conjugates

Nature rarely joins nucleic acids to proteins covalently, yet synthetic protein-nucleic acid conjugates enable diverse applications from nanotechnology to therapeutics, which require or benefit from defined, stable, one-to-one linkages of known geometry. Here I evaluate biochemical routes to such conjugates against fou...

A Robust DIA-Based Platform for Large-Scale Plasma Glycoproteomics and Biomarker Discovery

The platform successfully identified glycoform-specific changes and distinguished between noncancer individuals and those with stage III/IV lung cancer, establishing a high-throughput DIA-based glycoproteomics workflow suitable for large-cohort studies to discover glycopeptide biomarker candidates.

Hepato- and Immunoprotective Dynamics of Standardised Andrographis paniculata Extract in a Murine Polymicrobial Sepsis Model

Background: Sepsis-associated liver injury drives mortality in polymicrobial sepsis yet lacks targeted adjunctive therapy. Andrographis paniculata (Burm. f.) Nees, known in Indonesia as sambiloto, yields andrographolide, a diterpene lactone that suppresses nuclear factor kappa B (NF-κB) and activates nuclear factor ery...

Structural biology of protein misfolding and aggregation: from disease to therapeutic innovations

LncFENIX restricts influenza A virus replication by impairing PDIA3-mediated oxidative folding of hemagglutinin

Influenza A virus (IAV) relies on host endoplasmic reticulum (ER) homeostasis machinery to support the oxidative folding and maturation of viral glycoproteins. Although long non-coding RNAs (lncRNAs) are increasingly recognized as critical regulators of host-virus interactions, whether host lncRNAs modulate ER protein-...

Plasma multi-omics reveals molecular remodeling and prognostic biomarkers in advanced breast cancer treated with trastuzumab deruxtecan

Paired plasma multi-omics revealed T-DXd-associated proteomic and metabolic remodeling and the molecular patterns and PFS-associated biomarkers are hypothesis-generating and require validation in larger independent cohorts.

Assembly-Pathway Regulation Dictates pH-Responsive Actuation in the R-Body Protein Machinery

Abstract Refractile bodies (R-bodies) are protein assemblies that form intracellular rolls and extend into spirals in response to environmental stimuli. Type 51 R-body assembles from four Reb proteins and undergoes rapid, repeatable ∼50-fold extension. Although R-bodies were described more than 70 years ago, the roles...

An Evolutionarily Conserved Ypk1/SGK1 Kinase Pathway Regulates the Unfolded Protein Response by Modulating the Ire1 Protein Abundance

This fundamental study provides genetic and biochemical evidence that the Ypk1/SGK1 signaling pathway promotes protein homeostasis by controlling Ire1/IRE1 protein abundance and identifies an evolutionarily conserved Ypk1/SGK1 signaling pathway that regulates the HAC1 and XBP1 mRNA splicing by modulating the Ire1 prote...

Lipid-associated architecture and divergent ammonium transport distinguish a bacterial Rhesus protein

Nitrification is a major process in the global nitrogen cycle, initiated by the oxidation of ammonia to nitrite. The ammonia-oxidising bacterium Nitrosomonas europaea catalyses this first and rate-limiting step and depends on ammonium both as an energy substrate and as a nitrogen source, making ammonium acquisition cen...

Gain and loss of acquired doxycycline resistance in Lactiplantibacillus plantarum : an adaptive laboratory evolution study

ABSTRACT Probiotics interact in a mutualistic way with humans. Exposure of probiotics to sublethal concentrations of commonly prescribed antibiotics can cause resistance to arise. Over 1,000 generations, we performed an adaptive laboratory evolution experiment to determine if the fitness of the probiotic Lactiplantibac...

Biochar mitigates dimethylarsenate-stimulated methane emissions mediated by DOM-microbe interactions in paddy soils

Introduction Paddy soils represent key biogeochemical interfaces linking arsenic (As) transformation and carbon (C) cycling. Dimethylarsenate (DMAs), a widespread organoarsenic contaminant in rice paddies, can alter methane (CH4) dynamics, but the underlying mechanisms based on dissolved organic matter (DOM)-microbe in...

Purification, Characterization, and In Vitro Safety Assessment of the Antimicrobial Peptide SAM-KB40 from Bacillus licheniformis SAM-D318

While the peptide shows promising in vitro dual antibacterial and toxin-neutralizing capacity, its high sensitivity to proteinase K limits oral application.