Two orthogonal systems for inducible transcriptional repression in the malaria parasite Plasmodium falciparum using bacterial repressor proteins are created, improving on previous attempts at transcriptional regulation by two orders of magnitude and outperforming gold standard translational/post-transcriptional regulat...
Pablo Cárdenas Ramírez, Sebastian Smick, S. Dey et al.· bioRxiv· 0 citations
Prot-LAMBDA is introduced, a PLM that explicitly incorporates spatial relationships by coupling residue embeddings with inter-residue contacts and LambdaFold, a lightweight distance-guided structure prediction framework that achieves performance comparable to ESMFold on proteins strictly non-redundant to the training d...
The highly modular network restricted horizontal gene transfer of ARGs, mitigated the enrichment of pathogenic antibiotic-resistant bacteria (PARBs) and occurrence of high-risk ARGs in MMB-CW, providing an optimization strategy for MMB-CW in view of treatment performance and ecological risk.
Lei Zhou, Jiawei Xie, Li-Ming Zhang et al.· Environmental Research· 0 citations
The application of quercetin in functional foods is severely limited by its poor aqueous solubility, chemical instability, and low oral bioaccessibility. This study investigated the synergistic effects of ultrasound pretreatment and λ-carrageenan (λ-CA) complexation on the stability and quercetin delivery efficiency of...
Regulation of walnut fruit maturation is crucial for delaying harvest and maintaining kernel quality. In this study, 'Qingxiang' walnut (Juglans regia L.) fruit were treated with 300 mg L-1 gibberellic acid (GA3) during 20-100 days after flowering (DAF) to assess its effects on fruit development, kernel nutrient compos...
Fan Yang, Fang-Zhi Wu, Si-Ke Wang et al.· Plant physiology and biochem...· 0 citations
Abstract Commercial Bacillus-based probiotics are widely used in poultry production, but their biological mechanisms of action in broiler chickens are still not fully understood. This study evaluated the effects of dietary supplementation with Bacillus-based commercial probiotics on the performance, meat and tibia trai...
V. Vu, Soo-Ki Kim, H. Bui et al.· Journal of Animal Science· 0 citations
Nacre supplementation was associated with improved performance in the Y-maze and novel object recognition tests, reduced amyloid-β deposition and Congo red-positive plaque burden, lower phosphorylated tau immunoreactivity, and fewer histologically degenerated hippocampal neurons.
Kanae Ohara, Heng Zhang, Hiroto Shibata et al.· Journal of Dietary Supplemen...· 0 citations
A unified view is provided of how genome structure is established, how it relates to function, and how its disruption contributes to tumorigenesis, suggesting that alterations in genome structure can, in some contexts, actively reshape oncogenic programs.
Rebecca G. Smith, Hannah M. Wilson, Kathleen L. Schiela et al.· Genes & Development· 0 citations
A comparative protein engineering strategy that integrates prior mutagenesis, bioinformatics, and structural insights to reprogram LC protease specificity is reported, establishing comparative protein engineering as an effective framework for retargeting botulinum neurotoxin proteases.
J. E. Garrido, G. S. Salcedo, Max W. Strul et al.· Cell Chemical Biology· 0 citations
A framework that overcomes the internalization barrier, broadening the target scope for therapeutic and diagnostic conjugates is established and extended BTR to programmed cell death ligand 1 (PD-L1) and an mRNA-display-derived FAP peptide, suggesting potential broad applicability.
Zi-Hao Wen, Meng-Xin Xu, Zi-Jun Yan et al.· Nature· 1 citation
Three commonly used types of neutralization assay were compared and strong correlations and significant differences in measured neutralization between the different assays were found, highlighting areas for more precise and harmonized terminology and particular assay elements that must be considered in efforts to harmo...
E. Stadler, F. Mordant, Matthew J. Gartner et al.· Journal of Virology· 0 citations
It is found that the MIC10 proteins Mic10, Mic26, and Mic27 strongly recruit cardiolipin at conserved positive loop motifs, driving oligomerization of these subunits and resulting in the stabilization of curvature in model membranes.
C. Brown, T. Wassenaar, Z. Freyberg et al.· Science Advances· 1 citation· ⚡1
A new machine-learning framework aims to improve the success rate of computational protein design while moving away from results that reproduce sequences found in nature.
