protein folding

Solution fold of HIV-1 Virus protein U cytoplasmic domain in the presence of DPC micelles

Soil Protists Consume Viruses to Drive Microbial Nutrient Cycling: Overlooked Trophic Pathways in Soil Food Web

Soil viruses are traditional top-down regulators that redistribute organic matter through host lysis and the viral shunt, implicitly positioning them as terminal sinks rather than active trophic components. Here, we show, for the first time, that soil viruses can also be direct prey for protists, establishing an over...

On the Structural Precision Required for Ligand-Specific Elastic Network Dynamics of AI Co-Folded GPCR Structures

Background/Objectives: AI-based protein–ligand co-folding methods such as Boltz-2 are able to reproduce experimentally determined ligand binding poses at G protein-coupled receptors (GPCRs) with increasing accuracy and precision. As these models advance, they raise several natural questions: assuming the correct placem...

The bromodomain and its contribution to human health

ReCAST: Single-Cell-Informed Tumour Microenvironment Profiling for Translational Prognostic Assessment in Esophageal Cancer

Background/Objectives: Single-cell transcriptomics provides detailed maps of the tumour microenvironment, but translating these maps to large clinical cohorts requires methods that can recover biologically meaningful cell states from routinely available bulk transcriptomes and establish whether these states provide inf...

Integrated Strategy for Sustainable Outdoor Photo-Fermentative Biohydrogen Production: Sequential Optimization, Repeated-Batch Operation, Modular Bioreactor Simulation, and Biomass Valorization

This study developed an integrated strategy for outdoor photo-fermentative biohydrogen production by Rhodopseudomonas pentothenatexigens KKU-SN1/1 through low-cost medium optimization, repeated-batch operation, modular bioreactor simulation, and biomass valorization. A sequential experimental design combining one-facto...

Associations of the single nucleotide polymorphism rs2228145 of the IL6R gene with features of the clinical course of hypertrophic cardiomyopathy

Introduction. The study of modifiable cardiometabolic risk factors (CMRFs) influencing the clinical phenotype of hypertrophic cardiomyopathy (HCM) is currently relevant. It is known that modifier genes can affect the course and progression of symptoms in HCM. At the same time, the analysis of associations between si...

Structural and functional insights into the recombinant PR-10 tomato allergen Sola l 4.02

Abstract Sola l 4.02 is a major tomato allergen of the PR-10 family and a Bet v 1 homolog implicated in pollen–food cross-reactivity, yet its molecular properties remain poorly defined. Here, we report the recombinant production and its comprehensive structural and functional characterization. Sequence and structural a...

ArcRefine improves de novo binder designs while preserving binding modes

De novo protein design now enables the creation of high-affinity binders from a target structure alone. However, current design methods face a trade-off between site control and success rate. Generative models such as RFdiffusion and BoltzGen place binders at chosen sites but often require large candidate pools. Halluc...

Biochemical classification and structural insights into snake venom proteins and peptides

Novel Microparticulation of Pea Protein: Structural Transformation, Enhanced Gelation, Dispersion Stability, and Off‐Taste Masking

Pea protein isolate (PPI) suffers from poor solubility, weak gel‐forming ability, and undesirable sensory attributes that limit its wider adoption in plant‐based food products. This study reports a novel three‐stage process for producing plant protein microparticulate by combining pH shift, high‐pressure homogeniza...

Photoactive Chiral Nanoneedles for Site-Selective Cleavage and Broad-Spectrum Inactivation of Viruses

Respiratory viruses spread through aerosols and contaminated surfaces, yet masks, respirators, and filters only trap pathogens without inactivating them, turning used protective equipment into a secondary transmission source. Here, we report chiral ZnO nanoneedles (L-NNs or D-NNs), stabilized by L- or D-phenylalanine...