It is demonstrated that mutations in dfrA coding and upstream regulatory regions contribute to para-aminosalicylic acid resistance in Mtb clinical isolates by integrating genomic analysis with functional assays of thermal stability, promoter activity and drug susceptibility.
Rong Zeng, Najeeb Ullah, Jing Gu et al.· JAC-Antimicrobial Resistance· 0 citations
In donor-derived models, THBS1 knockdown in NLM cells reduced area-confluence trajectories, increased migration, and decreased collagen-rich matrix in 4-week spheroids, whereas THBS1 overexpression in DLM cells produced reciprocal changes; aggrecan was not consistently altered.
Shun-Jie Yang, Chen Hou, Hui Wang et al.· Frontiers in Cell and Develo...· 0 citations
Background: Visceral leishmaniasis caused by Leishmania donovani still depends on a small group of drugs whose value is diminished by toxicity, cost and resistance, so targets specific to the parasite remain attractive. The cytoplasmic poly(A)-binding protein (PABP) of Leishmania governs mRNA stability and translation,...
Haimn Abdulbassit Tawfiq, Ahmed Ali Mohammed, Yassir Mustafa Al Mulla Hummadi et al.· Al Mustansiriyah Journal of...· 0 citations
The study of macromolecular assembly formation is one of the most challenging tasks in computational modeling as it requires extensive exploration of conformational and configurational space, especially when driven by conformational transitions or folding instabilities, which are typically associated with high energy b...
Margherita Bini, Valentina Tozzini· Frontiers in Molecular Biosc...· 0 citations
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Protein engineering has evolved from modifying naturally occurring proteins toward modular, computational, and AI-assisted approaches for designing new molecular functions. Chimeric and fusion protein engineering has demonstrated that functional elements from different proteins can be reorganized into novel architectur...
The outstanding research questions of our BAG (encompassing 9 PIs) focus on proteins and their complexes involved in a variety of processes, ranging from cell homeostasis (from gene regulation to folding quality control, from energy metabolism to signal transduction) to communication and surface recognition (including...
Federico Forneris, Francesca Gasparini, Andrea Gottinger et al.· European Synchrotron Radiati...· 0 citations
Dengue virus (DENV) infection can result in outcomes ranging from subclinical infection to life-threatening illness, yet the antibody features mediating protection in humans remain poorly defined. Dengue virus envelope protein (E) forms homodimers on the virion surface and elicits both neutralizing and non-neutralizing...
Linker optimisation in targeted protein degradation remains largely empirical, and a computational method able to rank close analogues before synthesis would be valuable. Whether current methods can do so has not been tested against the smallest chemical difference that changes potency. Here two VHL-recruiting SHP2 deg...
A series of novel semisynthetic indole-fused taraxerone derivatives (2a–2f) were synthesised from taraxerone isolated from Rhizophora stylosa Griff. and characterised by 1H NMR,13C NMR, and HRMS analyses. Incorporation of the pharmacologically important indole moiety was aimed at enhancing protein-ligand interactions a...
Children in conflict zones endure chronic psychosocial stress with unclear biological consequences. We examined whether war-related caregiver-reported distress behaviors are associated with childhood anemia and whether systemic inflammation is consistent with a proposed statistical mediating pathway in Yemen, using a c...
Galal Faisal Albani, Nahid Ali, Khalil A. Saleh et al.· Conflict and Health· 0 citations
NEUROCODEX presents a revolutionary paradigm shift in neuroscience: the brain is not a computer, but rather the original algorithmic architect from which all artificial intelligence is merely a primitive derivation. This treatise demonstrates how biological systems achieve extraordinary energy efficiency (20 watts vers...
mohamed kamal arafa el-rakhawi· Zenodo (CERN European Organi...· 0 citations
Small heat shock proteins (sHSPs) are ATP-independent chaperones synthesized by cells in response to various stresses, including pathogen infection, to maintain cellular and protein homeostasis. However, their roles in shrimp innate immunity remain largely unknown. Here, we demonstrate that LvHSP10 is a key immune regu...
Pithiwat Maiket, Nutthapon Sangklai, Warumporn Yingsunthonwattana et al.· Fish and Shellfish Immunolog...· 0 citations
A new machine-learning framework aims to improve the success rate of computational protein design while moving away from results that reproduce sequences found in nature.
